Key features and details
- Rabbit polyclonal to SPON2
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SPON2 antibody
See all SPON2 primary antibodies
DescriptionRabbit polyclonal to SPON2
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
- ICC/IF: HaCaT cells. WB: Human liver lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab221649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 36 kDa.|
FunctionCell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (By similarity). Binds bacterial lipopolysaccharide (LPS).
Tissue specificityExpressed in normal lung tissue but not in lung carcinoma cell lines.
Sequence similaritiesContains 1 spondin domain.
Contains 1 TSP type-1 domain.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Differentially expressed in cancerous and non-cancerous lung cells 1 antibody
- DIL 1 antibody
- DIL-1 antibody
All lanes : Anti-SPON2 antibody (ab221649) at 1/250 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Predicted band size: 36 kDa
PFA-fixed, Triton X-100 permeabilized HaCaT (human keratinocyte cell line) cells stained for SPON2 (green) using ab221649 at 4 µg/ml in ICC/IF.
ab221649 has not yet been referenced specifically in any publications.