Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab221649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||1/250 - 1/500. Predicted molecular weight: 36 kDa.|
FunctionCell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (By similarity). Binds bacterial lipopolysaccharide (LPS).
Tissue specificityExpressed in normal lung tissue but not in lung carcinoma cell lines.
Sequence similaritiesContains 1 spondin domain.
Contains 1 TSP type-1 domain.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Differentially expressed in cancerous and non-cancerous lung cells 1 antibody
- DIL 1 antibody
- DIL-1 antibody
All lanes : Anti-SPON2 antibody (ab221649) at 1/250 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Predicted band size: 36 kDa
PFA-fixed, Triton X-100 permeabilized HaCaT (human keratinocyte cell line) cells stained for SPON2 (green) using ab221649 at 4 µg/ml in ICC/IF.
ab221649 has not yet been referenced specifically in any publications.