Anti-SPOP antibody (ab137537)
Key features and details
- Rabbit polyclonal to SPOP
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-SPOP antibody
See all SPOP primary antibodies -
Description
Rabbit polyclonal to SPOP -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Zebrafish, Xenopus tropicalis -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-223 of Human SPOP.
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Positive control
- WB: HepG2, mouse brain, transfected HEK-293T lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. |
Target
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Function
Inhibits IPF1/PDX1 transactivation of established target promoters, such as insulin, may be by recruiting a repressor complex (By similarity). In complex with CUL3, involved in ubiquitination of BMI1, H2AFY and DAXX, and probably also in ubiquitination and proteasomal degradation of Gli2 or Gli3. -
Tissue specificity
Widely expressed. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the Tdpoz family.
Contains 1 BTB (POZ) domain.
Contains 1 MATH domain. -
Domain
The MATH domain interacts with H2AFY and BMI1. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 530618 Cow
- Entrez Gene: 8405 Human
- Entrez Gene: 20747 Mouse
- Entrez Gene: 287643 Rat
- Entrez Gene: 394599 Xenopus tropicalis
- Entrez Gene: 100005514 Zebrafish
- Omim: 602650 Human
- SwissProt: Q0VCW1 Cow
see all -
Alternative names
- BTBD32 antibody
- HIB homolog 1 antibody
- Roadkill homolog 1 antibody
see all
Images
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All lanes : Anti-SPOP antibody (ab137537) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HepG2 nuclear extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 42 kDa10% SDS-PAGE
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Anti-SPOP antibody (ab137537) at 1/2000 dilution + Mouse brain lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 42 kDa
10% SDS PAGE -
All lanes : Anti-SPOP antibody (ab137537) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 42 kDa10% SDS-PAGE
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab137537 has been referenced in 16 publications.
- Zhu Q et al. SPOP Inhibition of Endometrial Carcinoma and Its Clinicopathological Relationship. Appl Bionics Biomech 2022:5721630 (2022). PubMed: 35465183
- Zhou Q et al. Neddylation inhibition induces glutamine uptake and metabolism by targeting CRL3SPOP E3 ligase in cancer cells. Nat Commun 13:3034 (2022). PubMed: 35641493
- Sun D et al. Evaluation of the Small-molecule BRD4 Degrader CFT-2718 in Small-cell Lung Cancer and Pancreatic Cancer Models. Mol Cancer Ther 20:1367-1377 (2021). PubMed: 34045230
- Wang D et al. ATM-phosphorylated SPOP contributes to 53BP1 exclusion from chromatin during DNA replication. Sci Adv 7:N/A (2021). PubMed: 34144977
- El Bezawy R et al. SPOP Deregulation Improves the Radiation Response of Prostate Cancer Models by Impairing DNA Damage Repair. Cancers (Basel) 12:N/A (2020). PubMed: 32512734