Anti-SPOP antibody (ab168619)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-SPOP antibody
See all SPOP primary antibodies -
DescriptionMouse polyclonal to SPOP
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Host speciesMouse
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Xenopus laevis, Zebrafish, Orangutan, Xenopus tropicalis
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Immunogen
Full length protein, corresponding to amino acids 1-374 of Human SPOP (NP_001007227.1).
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Positive control
- SPOP transfected 293T cell lysate; HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferpH: 7.20
Constituent: 99% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab168619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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FunctionInhibits IPF1/PDX1 transactivation of established target promoters, such as insulin, may be by recruiting a repressor complex (By similarity). In complex with CUL3, involved in ubiquitination of BMI1, H2AFY and DAXX, and probably also in ubiquitination and proteasomal degradation of Gli2 or Gli3.
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Tissue specificityWidely expressed.
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PathwayProtein modification; protein ubiquitination.
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Sequence similaritiesBelongs to the Tdpoz family.
Contains 1 BTB (POZ) domain.
Contains 1 MATH domain. -
DomainThe MATH domain interacts with H2AFY and BMI1.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 530618 Cow
- Entrez Gene: 8405 Human
- Entrez Gene: 20747 Mouse
- Entrez Gene: 779391 Xenopus laevis
- Entrez Gene: 394599 Xenopus tropicalis
- Entrez Gene: 100005514 Zebrafish
- Omim: 602650 Human
- SwissProt: Q0VCW1 Cow
see all -
Alternative names
- BTBD32 antibody
- HIB homolog 1 antibody
- Roadkill homolog 1 antibody
see all
Images
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Western blot - Anti-SPOP antibody (ab168619)All lanes : Anti-SPOP antibody (ab168619) at 1 µg/ml
Lane 1 : SPOP transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes :
Goat Anti-Mouse IgG (H&L)-HRP
at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 42 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-SPOP antibody (ab168619)Immunofluorescent analysis of HeLa cells labeling SPOP with ab168619 at 10 µg/ml.
Datasheets and documents
References
ab168619 has not yet been referenced specifically in any publications.
