Anti-SPOP antibody (ab168619)

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Overview

  • Product name

    Anti-SPOP antibody
    See all SPOP primary antibodies

  • Description

    Mouse polyclonal to SPOP

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Xenopus laevis, Zebrafish, Orangutan, Xenopus tropicalis

  • Immunogen

    Full length protein, corresponding to amino acids 1-374 of Human SPOP (NP_001007227.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • SPOP transfected 293T cell lysate; HeLa cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    pH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab168619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

Images

  • Western blot - Anti-SPOP antibody (ab168619)
    Western blot - Anti-SPOP antibody (ab168619)
    All lanes : Anti-SPOP antibody (ab168619) at 1 µg/ml

    Lane 1 : SPOP transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes :
    Goat Anti-Mouse IgG (H&L)-HRP
    at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 42 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SPOP antibody (ab168619)
    Immunocytochemistry/ Immunofluorescence - Anti-SPOP antibody (ab168619)
    Immunofluorescent analysis of HeLa cells labeling SPOP with ab168619 at 10 µg/ml.

References

This product has been referenced in:

  • Gong H  et al. APPBP2 enhances non-small cell lung cancer proliferation and invasiveness through regulating PPM1D and SPOP. EBioMedicine 44:138-149 (2019). Read more (PubMed: 31105033) »
See 1 Publication for this product

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