Anti-SPOP antibody (ab168619)
Key features and details
- Mouse polyclonal to SPOP
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPOP antibody
See all SPOP primary antibodies -
Description
Mouse polyclonal to SPOP -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Xenopus laevis, Zebrafish, Orangutan, Xenopus tropicalis -
Immunogen
Full length protein, corresponding to amino acids 1-374 of Human SPOP (NP_001007227.1).
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Positive control
- SPOP transfected 293T cell lysate; HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab168619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. | |
ICC/IF | Use a concentration of 10 µg/ml. |
Target
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Function
Inhibits IPF1/PDX1 transactivation of established target promoters, such as insulin, may be by recruiting a repressor complex (By similarity). In complex with CUL3, involved in ubiquitination of BMI1, H2AFY and DAXX, and probably also in ubiquitination and proteasomal degradation of Gli2 or Gli3. -
Tissue specificity
Widely expressed. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the Tdpoz family.
Contains 1 BTB (POZ) domain.
Contains 1 MATH domain. -
Domain
The MATH domain interacts with H2AFY and BMI1. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 530618 Cow
- Entrez Gene: 8405 Human
- Entrez Gene: 20747 Mouse
- Entrez Gene: 779391 Xenopus laevis
- Entrez Gene: 394599 Xenopus tropicalis
- Entrez Gene: 100005514 Zebrafish
- Omim: 602650 Human
- SwissProt: Q0VCW1 Cow
see all -
Alternative names
- BTBD32 antibody
- HIB homolog 1 antibody
- Roadkill homolog 1 antibody
see all
Images
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All lanes : Anti-SPOP antibody (ab168619) at 1 µg/ml
Lane 1 : SPOP transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes :
Goat Anti-Mouse IgG (H&L)-HRP
at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 42 kDa -
Immunofluorescent analysis of HeLa cells labeling SPOP with ab168619 at 10 µg/ml.
Protocols
Datasheets and documents
References (3)
ab168619 has been referenced in 3 publications.
- Gong H et al. APPBP2 enhances non-small cell lung cancer proliferation and invasiveness through regulating PPM1D and SPOP. EBioMedicine 44:138-149 (2019). PubMed: 31105033
- Li Y et al. SPOP Regulates The Biological Mechanism Of Ovarian Cancer Cells Through The Hh Signaling Pathway. Onco Targets Ther 12:9239-9248 (2019). PubMed: 31819473
- Adelaiye-Ogala R et al. Therapeutic Targeting of Sunitinib-Induced AR Phosphorylation in Renal Cell Carcinoma. Cancer Res 78:2886-2896 (2018). PubMed: 29572225