Anti-SPOP antibody (ab168619)

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Western blot - Anti-SPOP antibody (ab168619)
  • Immunocytochemistry/ Immunofluorescence - Anti-SPOP antibody (ab168619)

Key features and details

  • Mouse polyclonal to SPOP
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-SPOP antibody
    See all SPOP primary antibodies

  • Description

    Mouse polyclonal to SPOP

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Xenopus laevis, Zebrafish, Orangutan, Xenopus tropicalis

  • Immunogen

    Full length protein, corresponding to amino acids 1-374 of Human SPOP (NP_001007227.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • SPOP transfected 293T cell lysate; HeLa cells.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    pH: 7.4
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab168619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

Images

  • Western blot - Anti-SPOP antibody (ab168619)
    Western blot - Anti-SPOP antibody (ab168619)
    All lanes : Anti-SPOP antibody (ab168619) at 1 µg/ml

    Lane 1 : SPOP transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes :
    Goat Anti-Mouse IgG (H&L)-HRP
    at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 42 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SPOP antibody (ab168619)
    Immunocytochemistry/ Immunofluorescence - Anti-SPOP antibody (ab168619)
    Immunofluorescent analysis of HeLa cells labeling SPOP with ab168619 at 10 µg/ml.

References (3)

Publishing research using ab168619? Please let us know so that we can cite the reference in this datasheet.

ab168619 has been referenced in 3 publications.

  • Gong H  et al. APPBP2 enhances non-small cell lung cancer proliferation and invasiveness through regulating PPM1D and SPOP. EBioMedicine 44:138-149 (2019). PubMed: 31105033
  • Li Y  et al. SPOP Regulates The Biological Mechanism Of Ovarian Cancer Cells Through The Hh Signaling Pathway. Onco Targets Ther 12:9239-9248 (2019). PubMed: 31819473
  • Adelaiye-Ogala R  et al. Therapeutic Targeting of Sunitinib-Induced AR Phosphorylation in Renal Cell Carcinoma. Cancer Res 78:2886-2896 (2018). PubMed: 29572225

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