Key features and details
- Mouse polyclonal to SPOP
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SPOP antibody
See all SPOP primary antibodies
DescriptionMouse polyclonal to SPOP
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Xenopus laevis, Zebrafish, Orangutan, Xenopus tropicalis
- SPOP transfected 293T cell lysate; HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.4
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab168619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionInhibits IPF1/PDX1 transactivation of established target promoters, such as insulin, may be by recruiting a repressor complex (By similarity). In complex with CUL3, involved in ubiquitination of BMI1, H2AFY and DAXX, and probably also in ubiquitination and proteasomal degradation of Gli2 or Gli3.
Tissue specificityWidely expressed.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the Tdpoz family.
Contains 1 BTB (POZ) domain.
Contains 1 MATH domain.
DomainThe MATH domain interacts with H2AFY and BMI1.
- Information by UniProt
- BTBD32 antibody
- HIB homolog 1 antibody
- Roadkill homolog 1 antibody
All lanes : Anti-SPOP antibody (ab168619) at 1 µg/ml
Lane 1 : SPOP transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
All lanes :
Goat Anti-Mouse IgG (H&L)-HRP
at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 42 kDa
Immunofluorescent analysis of HeLa cells labeling SPOP with ab168619 at 10 µg/ml.
ab168619 has been referenced in 3 publications.
- Gong H et al. APPBP2 enhances non-small cell lung cancer proliferation and invasiveness through regulating PPM1D and SPOP. EBioMedicine 44:138-149 (2019). PubMed: 31105033
- Li Y et al. SPOP Regulates The Biological Mechanism Of Ovarian Cancer Cells Through The Hh Signaling Pathway. Onco Targets Ther 12:9239-9248 (2019). PubMed: 31819473
- Adelaiye-Ogala R et al. Therapeutic Targeting of Sunitinib-Induced AR Phosphorylation in Renal Cell Carcinoma. Cancer Res 78:2886-2896 (2018). PubMed: 29572225