Overview

  • Product name
    Anti-SPOP antibody
    See all SPOP primary antibodies
  • Description
    Mouse polyclonal to SPOP
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Xenopus laevis, Zebrafish, Orangutan, Xenopus tropicalis
  • Immunogen

    Full length protein, corresponding to amino acids 1-374 of Human SPOP (NP_001007227.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • SPOP transfected 293T cell lysate; HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab168619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

Images

  • Western blot - Anti-SPOP antibody (ab168619)
    Western blot - Anti-SPOP antibody (ab168619)
    All lanes : Anti-SPOP antibody (ab168619) at 1 µg/ml

    Lane 1 : SPOP transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes :
    Goat Anti-Mouse IgG (H&L)-HRP
    at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 42 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SPOP antibody (ab168619)
    Immunocytochemistry/ Immunofluorescence - Anti-SPOP antibody (ab168619)
    Immunofluorescent analysis of HeLa cells labeling SPOP with ab168619 at 10 µg/ml.

References

ab168619 has not yet been referenced specifically in any publications.

Publishing research using ab168619? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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