Key features and details
- Rabbit polyclonal to SPPL2a
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SPPL2a antibody
DescriptionRabbit polyclonal to SPPL2a
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- WB: Jurkat whole cell lysate. ICC/IF: HeLa cells. IHC-P: Human prostate cancer tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200.|
|WB||1/2000 - 1/5000. Predicted molecular weight: 58 kDa.|
RelevanceThis protein, which may act as an intramembrane protease, contains an N-terminal protease-associated (PA) domain, several transmembrane regions, a hydrophilic loop, conservative sequences around the first and second aspartate residues, and an invariant PAL motif near the C terminus.
Cellular localizationCell Membrane; multi-pass membrane protein
- EC 3.4.99 antibody
- IMP3 antibody
- Intramembrane protease 3 antibody
Anti-SPPL2a antibody (ab237691) at 1/2000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 58 kDa
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SPPL2a (Green) using ab237691 at 1/100 dilution in ICC/IF analysis, followed by Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
Paraffin-embedded human prostate cancer tissue stained for SPPL2a using ab237691 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237691 has not yet been referenced specifically in any publications.