Anti-SPPL2a antibody (ab237691)
Key features and details
- Rabbit polyclonal to SPPL2a
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPPL2a antibody -
Description
Rabbit polyclonal to SPPL2a -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human SPPL2a aa 1-200.
Database link: Q8TCT8 -
Positive control
- WB: Jurkat whole cell lysate. ICC/IF: HeLa cells. IHC-P: Human prostate cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity greater than 95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/200.
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IHC-P |
1/20 - 1/200.
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WB |
1/2000 - 1/5000. Predicted molecular weight: 58 kDa.
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Notes |
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ICC/IF
1/50 - 1/200. |
IHC-P
1/20 - 1/200. |
WB
1/2000 - 1/5000. Predicted molecular weight: 58 kDa. |
Target
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Relevance
This protein, which may act as an intramembrane protease, contains an N-terminal protease-associated (PA) domain, several transmembrane regions, a hydrophilic loop, conservative sequences around the first and second aspartate residues, and an invariant PAL motif near the C terminus. -
Cellular localization
Cell Membrane; multi-pass membrane protein -
Database links
- Entrez Gene: 84888 Human
- Omim: 608238 Human
- SwissProt: Q8TCT8 Human
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Alternative names
- EC 3.4.99 antibody
- IMP3 antibody
- Intramembrane protease 3 antibody
see all
Images
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Anti-SPPL2a antibody (ab237691) at 1/2000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 58 kDa -
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SPPL2a (Green) using ab237691 at 1/100 dilution in ICC/IF analysis, followed by Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
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Paraffin-embedded human prostate cancer tissue stained for SPPL2a using ab237691 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab237691 has not yet been referenced specifically in any publications.