Key features and details
- Rabbit polyclonal to SPR - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SPR antibody - C-terminal
See all SPR primary antibodies
DescriptionRabbit polyclonal to SPR - C-terminal
SpecificityDetects endogenous levels of total SPR protein.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human SPR (C terminal).
Database link: P35270
- Human chromaffin cell tumor tissue and lovo cell lysates. Human breast cancer tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab198716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 28 kDa.|
|IHC-P||1/50 - 1/200.|
FunctionCatalyzes the final one or two reductions in tetra-hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin.
Involvement in diseaseDefects in SPR are the cause of dystonia DOPA-responsive due to sepiapterin reductase deficiency (DRDSPRD) [MIM:612716]. In the majority of cases, patients manifest progressive psychomotor retardation, dystonia and spasticity. Cognitive anomalies are also often present. The disease is due to severe dopamine and serotonin deficiencies in the central nervous system caused by a defect in BH4 synthesis. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures.
Sequence similaritiesBelongs to the sepiapterin reductase family.
modificationsIn vitro phosphorylation of Ser-213 by CaMK2 does not change kinetic parameters.
- Information by UniProt
- OTTHUMP00000160199 antibody
- SDR38C1 antibody
- Sepiapterin reductase (7,8 dihydrobiopterin:NADP+ oxidoreductase) antibody
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling SPR with ab198716 at 1/40 dilution.
All lanes : Anti-SPR antibody - C-terminal (ab198716) at 1/1500 dilution
Lane 1 : Human chromaffin cell tumor tissue lysate
Lane 2 : Lovo cell lysate
Lysates/proteins at 40 µg per lane.
Developed using the ECL technique.
Predicted band size: 28 kDa
Exposure time: 10 seconds
ab198716 has not yet been referenced specifically in any publications.