Overview

  • Product name

    Anti-SPR antibody [EPR9290]
    See all SPR primary antibodies
  • Description

    Rabbit monoclonal [EPR9290] to SPR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human SPR (UniProt: P35270).

  • Positive control

    • A549, MCF7, HepG2 and HeLa whole cell lysate (ab150035); Human thyroid gland carcinoma tissue; HeLa and MCF7 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Catalyzes the final one or two reductions in tetra-hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin.
  • Involvement in disease

    Defects in SPR are the cause of dystonia DOPA-responsive due to sepiapterin reductase deficiency (DRDSPRD) [MIM:612716]. In the majority of cases, patients manifest progressive psychomotor retardation, dystonia and spasticity. Cognitive anomalies are also often present. The disease is due to severe dopamine and serotonin deficiencies in the central nervous system caused by a defect in BH4 synthesis. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures.
  • Sequence similarities

    Belongs to the sepiapterin reductase family.
  • Post-translational
    modifications

    In vitro phosphorylation of Ser-213 by CaMK2 does not change kinetic parameters.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • OTTHUMP00000160199 antibody
    • SDR38C1 antibody
    • Sepiapterin reductase (7,8 dihydrobiopterin:NADP+ oxidoreductase) antibody
    • Sepiapterin reductase antibody
    • Short chain dehydrogenase/reductase family 38C, member 1 antibody
    • SPR antibody
    • SPRE_HUMAN antibody
    see all

Images

  • All lanes : Anti-SPR antibody [EPR9290] (ab157194) at 1/1000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 28 kDa

  • ab157194 showing +ve staining in Human normal pancreas.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab157194 showing +ve staining in Human heart.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab157194 showing +ve staining in Human endometrial carcinoma.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab157194 showing +ve staining in Human colonic adenocarcinoma.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow cytometic analysis of permeabilized MCF7 cells labeling TPST2 with ab157194 at 1/100 (red) or a rabbit IgG (negative) (green).
  • Immunofluorescent analysis of HeLa cells labeling TPST2 with ab157194 at 1/100 dilution.
  • Immunohistochemical analysis of paraffin-eEmbedded Human thyroid gland carcinoma tissue labeling TPST2 with ab157194 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Detection of TPST2 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab157194 at 1/10 dilution.

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Rabbit Tissue lysate - whole (brain)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
0.2 µg
Treatment
hypoxic 40 minutes at E25
Specification
brain

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Submitted Jan 25 2018

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