Key features and details
- Rabbit polyclonal to SPRED1
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SPRED1 antibody
See all SPRED1 primary antibodies
DescriptionRabbit polyclonal to SPRED1
Tested applicationsSuitable for: IHC-P, Flow Cyt, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant full length protein corresponding to Human SPRED1 aa 1-444.
MSEETATSDNDNSYARVRAVVMTRDDSSGGWLPLGGSGLSSVTVFKVPHQ EENGCADFFIRGERLRDKMVVLECMLKKDLIYNKVTPTFHHWKIDDKKFG LTFQSPADARAFDRGIRRAIEDISQGCPESKNEAEGADDLQANEEDSSSS LVKDHLFQQETVVTSEPYRSSNIRPSPFEDLNARRVYMQSQANQITFGQP GLDIQSRSMEYVQRQISKECGSLKSQNRVPLKSIRHVSFQDEDEIVRINP RDILIRRYADYRHPDMWKNDLERDDADSSIQFSKPDSKKSDYLYSCGDET KLSSPKDSVVFKTQPSSLKIKKSKRRKEDGERSRCVYCQERFNHEENVRG KCQDAPDPIKRCIYQVSCMLCAESMLYHCMSDSEGDFSDPCSCDTSDDKF CLRWLALVALSFIVPCMCCYVPLRMCHRCGEACGCCGGKHKAAG
Database link: Q7Z699
- Human liver tissue, Jurkat cells, Jurkat cell lysate.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191278 in the following tested applications.
|IHC-P||1/75. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000. Predicted molecular weight: 50 kDa.|
FunctionTyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow.
Tissue specificityWeakly expressed in embryonic cell line (HEK-293).
Involvement in diseaseDefects in SPRED1 are the cause of Legius syndrome (LEGIUSS) [MIM:611431]. It is a disorder characterized mainly by cafe au lait macules without neurofibromas or other tumor manifestations of neurofibromatosis type 1, axillary freckling, and macrocephaly. Additional clinical manifestations include Noonan-like facial dysmorphism, lipomas, learning disabilities and attention deficit-hyperactivity.
Sequence similaritiesContains 1 KBD domain.
Contains 1 SPR (sprouty) domain.
Contains 1 WH1 domain.
modificationsPhosphorylated on tyrosine.
Cellular localizationCell membrane. Membrane > caveola. Nucleus. Localized in cholesterol-rich membrane raft/caveola fractions.
- Information by UniProt
- EVH1 domain-containing protein 1 antibody
- EVH1/Sprouty domain containing protein antibody
- FLJ33903 antibody
Anti-SPRED1 antibody (ab191278) at 1/1000 dilution + Jurkat cell lysate at 35 µg
Predicted band size: 50 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, labeling SPRED1 with ab191278 at 1/75 dilution.
Flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram), labeling SPRED1 with ab191278 at 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab191278 has not yet been referenced specifically in any publications.