Key features and details
- Rabbit polyclonal to SPRED1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SPRED1 antibody
See all SPRED1 primary antibodies
DescriptionRabbit polyclonal to SPRED1
Specificityab77079 is predicted to have no cross-reactivity to SPRED2 or SPRED3.
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic 14 amino acid peptide from near the center of human SPRED1 (NP_689807).
- Human brain tissue lysate. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: A549.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab77079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionTyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow.
Tissue specificityWeakly expressed in embryonic cell line (HEK-293).
Involvement in diseaseDefects in SPRED1 are the cause of Legius syndrome (LEGIUSS) [MIM:611431]. It is a disorder characterized mainly by cafe au lait macules without neurofibromas or other tumor manifestations of neurofibromatosis type 1, axillary freckling, and macrocephaly. Additional clinical manifestations include Noonan-like facial dysmorphism, lipomas, learning disabilities and attention deficit-hyperactivity.
Sequence similaritiesContains 1 KBD domain.
Contains 1 SPR (sprouty) domain.
Contains 1 WH1 domain.
modificationsPhosphorylated on tyrosine.
Cellular localizationCell membrane. Membrane > caveola. Nucleus. Localized in cholesterol-rich membrane raft/caveola fractions.
- Information by UniProt
- EVH1 domain-containing protein 1 antibody
- EVH1/Sprouty domain containing protein antibody
- FLJ33903 antibody
Lane 1 : Anti-SPRED1 antibody (ab77079) at 1 µg/ml
Lane 2 : Anti-SPRED1 antibody (ab77079) at 2 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
IHC image of ab77079 staining in human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77079, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab77079 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab77079 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of Spred1 in Human Brain cells using ab77079 at 20 ug/ml.
ab77079 has been referenced in 6 publications.
- Susanto A et al. Spred negatively regulates lens growth by modulating epithelial cell proliferation and fiber differentiation. Exp Eye Res 178:160-175 (2019). PubMed: 30290165
- D'Souza RF et al. Divergent effects of cold water immersion versus active recovery on skeletal muscle fiber type and angiogenesis in young men. Am J Physiol Regul Integr Comp Physiol 314:R824-R833 (2018). PubMed: 29466686
- Wang L et al. miR-126 Regulation of Angiogenesis in Age-Related Macular Degeneration in CNV Mouse Model. Int J Mol Sci 17:N/A (2016). WB ; Mouse . PubMed: 27338342
- Ji JS et al. Inhibition of microRNA-126 promotes the expression of Spred1 to inhibit angiogenesis in hepatocellular carcinoma after transcatheter arterial chemoembolization: in vivo study. Onco Targets Ther 9:4357-67 (2016). IHC-P . PubMed: 27499630
- Cao WJ et al. Therapeutic Angiogenesis by Ultrasound-Mediated MicroRNA-126-3p Delivery. Arterioscler Thromb Vasc Biol 35:2401-11 (2015). PubMed: 26381870
- Sharma SB et al. MicroRNAs 206 and 21 cooperate to promote RAS-extracellular signal-regulated kinase signaling by suppressing the translation of RASA1 and SPRED1. Mol Cell Biol 34:4143-64 (2014). WB ; Human . PubMed: 25202123