Overview

  • Product name

    Anti-SPRED1 antibody [M23-P2G3]
    See all SPRED1 primary antibodies
  • Description

    Mouse monoclonal [M23-P2G3] to SPRED1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human SPRED1 aa 400 to the C-terminus.

  • Positive control

    • This antibody gave a positive signal in the following lysates: HeLa whole cell, Jurkat whole cell, human brain tissue, mouse brain tissue, rat brain tissue
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab64740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 10 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

Target

  • Function

    Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow.
  • Tissue specificity

    Weakly expressed in embryonic cell line (HEK-293).
  • Involvement in disease

    Defects in SPRED1 are the cause of Legius syndrome (LEGIUSS) [MIM:611431]. It is a disorder characterized mainly by cafe au lait macules without neurofibromas or other tumor manifestations of neurofibromatosis type 1, axillary freckling, and macrocephaly. Additional clinical manifestations include Noonan-like facial dysmorphism, lipomas, learning disabilities and attention deficit-hyperactivity.
  • Sequence similarities

    Contains 1 KBD domain.
    Contains 1 SPR (sprouty) domain.
    Contains 1 WH1 domain.
  • Post-translational
    modifications

    Phosphorylated on tyrosine.
  • Cellular localization

    Cell membrane. Membrane > caveola. Nucleus. Localized in cholesterol-rich membrane raft/caveola fractions.
  • Information by UniProt
  • Database links

  • Alternative names

    • EVH1 domain-containing protein 1 antibody
    • EVH1/Sprouty domain containing protein antibody
    • FLJ33903 antibody
    • hSpred 1 antibody
    • hSpred1 antibody
    • NFLS antibody
    • PPP1R147 antibody
    • protein phosphatase 1 regulatory subunit 147 antibody
    • SPRE1_HUMAN antibody
    • SPRED 1 antibody
    • Spred-1 antibody
    • spred1 antibody
    • Sprouty related EVH1 domain containing 1 antibody
    • sprouty related EVH1 domain containing protein 1 antibody
    • Sprouty related protein 1 with EVH 1 domain antibody
    • Sprouty-related antibody
    • Suppressor of Ras/MAPK activation antibody
    see all

Images

  • All lanes : Anti-SPRED1 antibody [M23-P2G3] (ab64740) at 10 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)
    Lane 4 : Brain (Rat) Tissue Lysate
    Lane 5 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa


    Exposure time: 30 seconds

References

This product has been referenced in:

  • Pasmant E  et al. SPRED1, a RAS MAPK pathway inhibitor that causes Legius syndrome, is a tumour suppressor downregulated in paediatric acute myeloblastic leukaemia. Oncogene N/A:N/A (2014). Read more (PubMed: 24469042) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

Thanks for your call today and for letting us know about the trouble with these antibodies. As we discussed, I am sending two free of charge replacements that should arrive tomorrow on the order ***. Please keep me updated on how these work in your blots. I look forward to hearing from you, and let me know if you have any questions or if there is anything else that we can do for you.

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