Anti-SPRED2 antibody (ab153700)
Key features and details
- Rabbit polyclonal to SPRED2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPRED2 antibody -
Description
Rabbit polyclonal to SPRED2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment, corresponding to a region within acids 79-417 of Human SPRED2 (UniProt: Q7Z698).
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Positive control
- 293T and A431 whole cell lysates; Human Breast carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 48 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 48 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. -
Cellular localization
Cell Membrane - peripheral membrane protein and Cytoplasmic -
Database links
- Entrez Gene: 200734 Human
- Entrez Gene: 114716 Mouse
- Entrez Gene: 305539 Rat
- Omim: 609292 Human
- SwissProt: Q7Z698 Human
- SwissProt: Q924S7 Mouse
- SwissProt: Q3C2P8 Rat
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Alternative names
- C79158 antibody
- FLJ21897 antibody
- FLJ31917 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab153700 has been referenced in 5 publications.
- Chen D et al. MicroRNA-218 aggravates H2O2-induced damage in PC12 cells via spred2-mediated autophagy. Exp Ther Med 22:1352 (2021). PubMed: 34659498
- Hu L et al. LncRNA TUG1 reverses LPS-induced cell apoptosis and inflammation of macrophage via targeting MiR-221-3p/SPRED2 axis. Biosci Biotechnol Biochem 84:2458-2465 (2020). PubMed: 32841583
- Susanto A et al. Spred negatively regulates lens growth by modulating epithelial cell proliferation and fiber differentiation. Exp Eye Res 178:160-175 (2019). PubMed: 30290165
- Rusan M et al. Suppression of Adaptive Responses to Targeted Cancer Therapy by Transcriptional Repression. Cancer Discov 8:59-73 (2018). PubMed: 29054992
- Zhao G et al. Negative regulation of lens fiber cell differentiation by RTK antagonists Spry and Spred. Exp Eye Res 170:148-159 (2018). WB . PubMed: 29501879