Product nameAnti-Sprouty 1/Spry-1 antibody
DescriptionRabbit polyclonal to Sprouty 1/Spry-1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Macaque monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human Sprouty 1/Spry-1 aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- WB: Human liver and fetal liver tissue lysates. ICC/IF: methanol fixed HeLa cells. IHC-P: Human normal liver tissue.
Previously labelled as Sprouty 1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab111523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).|
FunctionMay function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis.
Sequence similaritiesBelongs to the sprouty family.
Contains 1 SPR (sprouty) domain.
DomainThe Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
Cellular localizationCytoplasm. Membrane. Found in the cytoplasm in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF.
- Information by UniProt
- hSPRY1 antibody
- Protein sprouty homolog 1 antibody
- Sprouty 1 antibody
All lanes : Anti-Sprouty 1/Spry-1 antibody (ab111523) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Liver (Human) Tissue Lysate - fetal normal tissue (ab29890)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Additional bands at: 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
ICC/IF image of ab111523 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab111523 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of Sprouty 1/Spry-1 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA based pH 9.0 solution (epitope retrieval solution 2) for 20 mins. The section was then incubated with ab111523, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times