Anti-Sprouty 4/Spry-4 antibody (ab59785)
Key features and details
- Rabbit polyclonal to Sprouty 4/Spry-4
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Sprouty 4/Spry-4 antibody
See all Sprouty 4/Spry-4 primary antibodies -
Description
Rabbit polyclonal to Sprouty 4/Spry-4 -
Host species
Rabbit -
Specificity
This antibody reacts specifically with human 32 kDa Sprouty 4/Spry-4 protein.
-
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chicken, Cow -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
-
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab59785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use at an assay dependent concentration.
|
Notes |
---|
WB
Use at an assay dependent concentration. |
Target
-
Function
Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. -
Sequence similarities
Belongs to the sprouty family.
Contains 1 SPR (sprouty) domain. -
Domain
The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles. -
Cellular localization
Cytoplasm. Cell projection > ruffle membrane. - Information by UniProt
-
Database links
- Entrez Gene: 427643 Chicken
- Entrez Gene: 504593 Cow
- Entrez Gene: 81848 Human
- Entrez Gene: 24066 Mouse
- Omim: 607984 Human
- SwissProt: A2VDU1 Cow
- SwissProt: Q9C004 Human
- SwissProt: Q9WTP2 Mouse
see all -
Alternative names
- Protein sprouty homolog 4 antibody
- Sprouty 4 antibody
- Sprouty homolog 4 antibody
see all
Datasheets and documents
-
Datasheet download
References (5)
ab59785 has been referenced in 5 publications.
- Guo J et al. SPRY4 suppresses proliferation and induces apoptosis of colorectal cancer cells by repressing oncogene EZH2. Aging (Albany NY) 13:11665-11677 (2021). PubMed: 33879635
- Chai Z et al. miR-1908 as a novel prognosis marker of glioma via promoting malignant phenotype and modulating SPRY4/RAF1 axis. Oncol Rep 38:2717-2726 (2017). PubMed: 29048686
- Edmonds MD et al. MicroRNA-31 initiates lung tumorigenesis and promotes mutant KRAS-driven lung cancer. J Clin Invest 126:349-64 (2016). PubMed: 26657862
- Lee JY et al. Novel Function of Sprouty4 as a Regulator of Stemness and Differentiation of Embryonic Stem Cells. Dev Reprod 20:171-7 (2016). WB . PubMed: 27660833
- Barbáchano A et al. SPROUTY-2 and E-cadherin regulate reciprocally and dictate colon cancer cell tumourigenicity. Oncogene 29:4800-13 (2010). WB ; Human . PubMed: 20543868