Overview

  • Product name

    Anti-Sprouty 4/Spry-4 antibody
    See all Sprouty 4/Spry-4 primary antibodies
  • Description

    Rabbit polyclonal to Sprouty 4/Spry-4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Cow
  • Immunogen

    Recombinant fragment within Human Sprouty 4/Spry-4 (internal sequence). The exact sequence is proprietary.
    Database link: Q9C004

  • Positive control

    • WB: Mouse brain lysate; NT2/D1 whole cell lysate. IHC-P: U87 xenograft tissue; Mouse duodenum tissue.
  • General notes

    Previously labelled as Sprouty 4.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 33 kDa.

Target

Images

  • Anti-Sprouty 4/Spry-4 antibody (ab228712) at 1/1000 dilution + Mouse brain lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 33 kDa



    12% SDS-PAGE gel.

  • Paraffin-embedded U87 xenograft tissue stained for Sprouty 4/Spry-4 using ab228712 at 1/500 dilution in immunohistochemical analysis.

  • Anti-Sprouty 4/Spry-4 antibody (ab228712) at 1/500 dilution + NT2/D1 (human malignant pluripotent embryonic carcinoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 33 kDa



    12% SDS-PAGE gel.

  • Paraffin-embedded mouse duodenum tissue stained for Sprouty 4/Spry-4 using ab228712 at 1/500 dilution in immunohistochemical analysis.

References

ab228712 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab228712.
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