Anti-Sprouty 4/Spry-4 antibody (ab262874)
Key features and details
- Rabbit polyclonal to Sprouty 4/Spry-4
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Sprouty 4/Spry-4 antibody
See all Sprouty 4/Spry-4 primary antibodies -
Description
Rabbit polyclonal to Sprouty 4/Spry-4 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Sprouty 4/Spry-4 aa 1-100.
Database link: A0A0C4DFS6 -
Positive control
- ICC/IF: PC-3 cells; WB: U-251 MG cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab262874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
|
WB |
Use a concentration of 0.4 µg/ml.
|
Notes |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
WB
Use a concentration of 0.4 µg/ml. |
Target
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Function
Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. -
Sequence similarities
Belongs to the sprouty family.
Contains 1 SPR (sprouty) domain. -
Domain
The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles. -
Cellular localization
Cytoplasm. Cell projection > ruffle membrane. - Information by UniProt
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Database links
- Entrez Gene: 81848 Human
- Omim: 607984 Human
- SwissProt: Q9C004 Human
- Unigene: 323308 Human
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Alternative names
- Protein sprouty homolog 4 antibody
- Sprouty 4 antibody
- Sprouty homolog 4 antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab262874 has not yet been referenced specifically in any publications.