Anti-Sprouty 4/Spry-4 antibody (ab7513)
Key features and details
- Rabbit polyclonal to Sprouty 4/Spry-4
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Sprouty 4/Spry-4 antibody
See all Sprouty 4/Spry-4 primary antibodies -
Description
Rabbit polyclonal to Sprouty 4/Spry-4This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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Host species
Rabbit -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Chimpanzee
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Immunogen
Synthetic peptide corresponding to Human Sprouty 4/Spry-4 aa 283-299 conjugated to keyhole limpet haemocyanin.
Sequence:CKAASGDAKTSRPDKPF
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General notes
Previously labelled as Sprouty 4.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | ||
| WB | (1) |
| Notes |
|---|
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested by Western blot against cell lysates. We believe that Sprouty 4 is represented by the doublet at ~ 35kDa. We believe that this protein may require IP before Western blotting, corresponding to results seen in Sasaki et al. We have not yet succeeded in peptide blocking the bands detected with this antibody.
Target
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Function
Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. -
Sequence similarities
Belongs to the sprouty family.
Contains 1 SPR (sprouty) domain. -
Domain
The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles. -
Cellular localization
Cytoplasm. Cell projection > ruffle membrane. - Information by UniProt
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Database links
- Entrez Gene: 504593 Cow
- Entrez Gene: 81848 Human
- Entrez Gene: 24066 Mouse
- Omim: 607984 Human
- SwissProt: A2VDU1 Cow
- SwissProt: Q9C004 Human
- SwissProt: Q9WTP2 Mouse
- Unigene: 323308 Human
see all -
Alternative names
- Protein sprouty homolog 4 antibody
- Sprouty 4 antibody
- Sprouty homolog 4 antibody
see all
Images
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Western blot - Anti-Sprouty 4/Spry-4 antibody (ab7513)
Western blot using ab7513 at 1/500.
Lane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: Jurkat
Lane 5: 293Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time: 2minutes.
Lysates at 5µg per lane.
We believe that CTCF is one of the bands seen in the pair of bands between 25 and 37kD.Western blot using ab7513 at 1/500.
Lane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: Jurkat
Lane 5: 293Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time: 2minutes.
Lysates at 5µg per lane.
We believe that CTCF is one of the bands seen in the pair of bands between 25 and 37kD. -
Western blot - Anti-Sprouty 4/Spry-4 antibody (ab7513)All lanes : Anti-Sprouty 4/Spry-4 antibody (ab7513) at 1/100 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : SW13 whole cell lysate
Lane 3 : C2C12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDaThe blot was incubated with ab7516 (1/100) overnight at 4°C.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sprouty 4/Spry-4 antibody (ab7513)ab7513 at 2.5 µg/ml staining human heart tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sprouty 4/Spry-4 antibody (ab7513)ab7513 staining tumour cells in human liver tissue. Brown color indicates presence of protein, blue color shows cell nuclei.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab7513 has been referenced in 3 publications.
- So WK et al. Sprouty4 mediates amphiregulin-induced down-regulation of E-cadherin and cell invasion in human ovarian cancer cells. Tumour Biol 37:9197-207 (2016). PubMed: 26768617
- Barbáchano A et al. SPROUTY-2 and E-cadherin regulate reciprocally and dictate colon cancer cell tumourigenicity. Oncogene 29:4800-13 (2010). WB ; Human . PubMed: 20543868
- Price KL et al. Microarray interrogation of human metanephric mesenchymal cells highlights potentially important molecules in vivo. Physiol Genomics 28:193-202 (2007). IHC-P ; Human . PubMed: 16985006