Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12127] to Sprouty 4/Spry-4
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-Sprouty 4/Spry-4 antibody [EPR12127]
See all Sprouty 4/Spry-4 primary antibodies
DescriptionRabbit monoclonal [EPR12127] to Sprouty 4/Spry-4
Tested applicationsSuitable for: IP, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
within Human Sprouty 4/Spry-4 aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: Q9C004
- WB: HepG2, MCF7, HeLa, and K562 cell lysates. IP: MCF7 cell lysate.
Previously labelled as Sprouty 4.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab176337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/10 - 1/100.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 33 kDa.|
FunctionSuppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras.
Sequence similaritiesBelongs to the sprouty family.
Contains 1 SPR (sprouty) domain.
DomainThe Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
Cellular localizationCytoplasm. Cell projection > ruffle membrane.
- Information by UniProt
- Protein sprouty homolog 4 antibody
- Sprouty 4 antibody
- Sprouty homolog 4 antibody
All lanes : Anti-Sprouty 4/Spry-4 antibody [EPR12127] (ab176337) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 33 kDa
Western blot analysis on immunoprecipitation pellet from MCF-7 cells lysate (lane 1) or 1XPBS (negative control, lane 2) using ab176337 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab176337 has been referenced in 1 publication.
- Tian Y et al. MicroRNA-181 serves an oncogenic role in breast cancer via the inhibition of SPRY4. Mol Med Rep 18:5603-5613 (2018). PubMed: 30365052