Overview

  • Product name

    Anti-Sprouty 4/Spry-4 antibody [EPR12127]
    See all Sprouty 4/Spry-4 primary antibodies
  • Description

    Rabbit monoclonal [EPR12127] to Sprouty 4/Spry-4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human Sprouty 4/Spry-4 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9C004

  • Positive control

    • HepG2, MCF7, HeLa, and K562 cell lysates. HeLa cells and permeabilized K562 cells.
  • General notes

     

     

    Previously labelled as Sprouty 4.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12127
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras.
    • Sequence similarities

      Belongs to the sprouty family.
      Contains 1 SPR (sprouty) domain.
    • Domain

      The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
    • Cellular localization

      Cytoplasm. Cell projection > ruffle membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Protein sprouty homolog 4 antibody
      • Sprouty 4 antibody
      • Sprouty homolog 4 antibody
      • sprouty homolog 4 (Drosophila) antibody
      • SPRY 4 antibody
      • Spry-4 antibody
      • Spry4 antibody
      • SPY4_HUMAN antibody
      see all

    Images

    • All lanes : Anti-Sprouty 4/Spry-4 antibody [EPR12127] (ab176337) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 33 kDa

    • Western blot analysis on immunoprecipitation pellet from MCF-7 cells lysate (lane 1) or 1XPBS (negative control, lane 2) using ab176337 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    This product has been referenced in:

    • Tian Y  et al. MicroRNA-181 serves an oncogenic role in breast cancer via the inhibition of SPRY4. Mol Med Rep 18:5603-5613 (2018). Read more (PubMed: 30365052) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab176337.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up