Recombinant Anti-Sprouty 4/Spry-4 antibody [EPR12127] (ab176337)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12127] to Sprouty 4/Spry-4
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Sprouty 4/Spry-4 antibody [EPR12127]
See all Sprouty 4/Spry-4 primary antibodies -
Description
Rabbit monoclonal [EPR12127] to Sprouty 4/Spry-4 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, MCF7, HeLa, and K562 cell lysates. IP: MCF7 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12127 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/10 - 1/100.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
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Notes |
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IP
1/10 - 1/100. |
WB
1/1000 - 1/10000. Predicted molecular weight: 33 kDa. |
Target
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Function
Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. -
Sequence similarities
Belongs to the sprouty family.
Contains 1 SPR (sprouty) domain. -
Domain
The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles. -
Cellular localization
Cytoplasm. Cell projection > ruffle membrane. - Information by UniProt
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Database links
- Entrez Gene: 81848 Human
- Entrez Gene: 24066 Mouse
- Entrez Gene: 291610 Rat
- Omim: 607984 Human
- SwissProt: Q9C004 Human
- SwissProt: Q9WTP2 Mouse
- Unigene: 323308 Human
- Unigene: 42038 Mouse
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Alternative names
- Protein sprouty homolog 4 antibody
- Sprouty 4 antibody
- Sprouty homolog 4 antibody
see all
Images
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All lanes : Anti-Sprouty 4/Spry-4 antibody [EPR12127] (ab176337) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 33 kDa -
Western blot analysis on immunoprecipitation pellet from MCF-7 cells lysate (lane 1) or 1XPBS (negative control, lane 2) using ab176337 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab176337 has been referenced in 1 publication.
- Tian Y et al. MicroRNA-181 serves an oncogenic role in breast cancer via the inhibition of SPRY4. Mol Med Rep 18:5603-5613 (2018). PubMed: 30365052