Overview

  • Product name

    Anti-Sprouty 4/Spry-4 (phospho Y75) antibody
    See all Sprouty 4/Spry-4 primary antibodies
  • Description

    Rabbit polyclonal to Sprouty 4/Spry-4 (phospho Y75)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Sprouty 4/Spry-4 aa 57-86 (internal sequence) conjugated to keyhole limpet haemocyanin.

  • Positive control

    • K-562 cell lysate. Human brain tissue.
  • General notes

    Previously labelled as Sprouty 4.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified through a protein A column, followed by peptide affinity purification.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 32 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras.
  • Sequence similarities

    Belongs to the sprouty family.
    Contains 1 SPR (sprouty) domain.
  • Domain

    The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
  • Cellular localization

    Cytoplasm. Cell projection > ruffle membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Protein sprouty homolog 4 antibody
    • Sprouty 4 antibody
    • Sprouty homolog 4 antibody
    • sprouty homolog 4 (Drosophila) antibody
    • SPRY 4 antibody
    • Spry-4 antibody
    • Spry4 antibody
    • SPY4_HUMAN antibody
    see all

Images

  • ab103114, at 1/50 dilution, staining Sprouty 4/Spry-4 in formalin-fixed, paraffin-embedded Human brain tissue by Immunohistochemistry followed by peroxidase conjugation of the secondary antibody and DAB detection.

  • Anti-Sprouty 4/Spry-4 (phospho Y75) antibody (ab103114) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size: 32 kDa

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
MCF7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 12 2013

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