Overview

  • Product name

    Anti-SPRR1a antibody
  • Description

    Rabbit polyclonal to SPRR1a
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Pig
  • Immunogen

    Synthetic peptide corresponding to Human SPRR1a aa 40-89 (C terminal).
    Sequence:

    PCHPKVPEPCHPKVPEPCQPKVPEPCQPKVPEPCPSTVTPAPAQQKTKQK


    Database link: P35321

  • Positive control

    • HT1080 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab125374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance

    The ability of neurons to regenerate an axon after injury is determined by both the surrounding environment and factors intrinsic to the damaged neuron. Neuronal SPRR1A is likely a significant contributor to successful nerve regeneration. The small proline-rich repeat protein 1A (SPRR1A) is not detectable in uninjured neurons but is induced by >60-fold after peripheral axonal damage being localized to injured neurons and axons. SPRR1A is one of a group of epithelial differentiation genes, including s100c and p21/waf, that are cocurrently induced in neurons by axotomy. Overexpressed SPRR1A colocalizes with F-actin in membrane ruffles and augments axonal outgrowth on a range of substrates. In axotomized sensory neurons, reduction of SPRR1A function restricts axonal outgrowth.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • 19 kDa pancornulin antibody
    • Cornifin A antibody
    • Cornifin alpha antibody
    • CornifinA antibody
    • Small proline rich protein 1A antibody
    • Small proline rich protein IA antibody
    • spr antibody
    • SPR IA antibody
    • SPRK antibody
    • SPRR 1a antibody
    • SPRR1A protein antibody
    see all

Images

  • Anti-SPRR1a antibody (ab125374) at 1 µg/ml + HT1080 cell lysate at 10 µg

    Predicted band size: 10 kDa



    Gel concentration: 10-20%
  • ab125374 staining SPRR1a in Human skin tissue by Immunohistochemistry (Frozen sections).

    Tissue was fixed with acetone and blocked with 1% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/300 in 1% BSA + 10% goat serum) for 16 hours at 4°C. An AlexaFluor®546-conjugated goat anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

    See Abreview

References

This product has been referenced in:

  • Han SB  et al. Postinjury Induction of Activated ErbB2 Selectively Hyperactivates Denervated Schwann Cells and Promotes Robust Dorsal Root Axon Regeneration. J Neurosci 37:10955-10970 (2017). Read more (PubMed: 28982707) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skin and spinal cord)
Permeabilization
Yes - 0.3% Triton-X100 in PBS
Specification
skin and spinal cord
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde

Herr Dr. Hendrik Wildner

Verified customer

Submitted Feb 28 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Yuan Cao

Verified customer

Submitted Jul 27 2012

Question
Answer

DISCOUNT CODE: *
Expiration date: *

I am very pleased to hear you would like to accept our offer and test ab125374 in IHC-P. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forIHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Question
Answer

Thank you for your phone call.


DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab125374 in IHC-P. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHC-Pand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Read More

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