Overview

  • Product name

    Anti-SPRR1b antibody
  • Description

    Rabbit polyclonal to SPRR1b
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Pig
  • Immunogen

    Synthetic peptide corresponding to amino acids 48-89 (PCHPKVPEPCHPKVPEPCQPKVPEPCHPKVPEPCPSIVTPAPAQQKTKQ K) of human SPRR1b (NP_003116).

  • Positive control

    • Jurkat whole cell lysate (ab7899).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab123237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    Cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane. Can function as both amine donor and acceptor in transglutaminase-mediated cross-linkage.
  • Tissue specificity

    Suprabasal layers of squamous-differentiated tissues such as epidermis, esophagus, tongue and trachea.
  • Sequence similarities

    Belongs to the cornifin (SPRR) family.
  • Developmental stage

    Expressed during differentiation of squamous cells.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 14.9 kDa pancornulin antibody
    • Cornifin antibody
    • Cornifin B antibody
    • Cornifin-B antibody
    • CornifinB antibody
    • GADD33 antibody
    • MGC61901 antibody
    • Small proline rich protein IB antibody
    • Small proline-rich protein IB antibody
    • SPR-IB antibody
    • SPR1B_HUMAN antibody
    • SPRIB antibody
    • SPRR1 antibody
    • SPRR1B antibody
    see all

Images

  • Immunohistochemistry (Frozen sections) analysis of mouse eyeball sections labelling  SPRR1b with ab123237 at 1:50. An Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:50) was used as the secondary antibody.

  • Anti-SPRR1b antibody (ab123237) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    Goat anti-Rabbit IgG HRP

    Predicted band size: 10 kDa



    Gel concentration: 10-20%.

References

This product has been referenced in:

  • Fang Z  et al. Long non-coding RNA MALAT-1 modulates metastatic potential of tongue squamous cell carcinomas partially through the regulation of small proline rich proteins. BMC Cancer 16:706 (2016). WB ; Human . Read more (PubMed: 27586393) »
See 1 Publication for this product

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