Key features and details
- Rabbit polyclonal to SPT5
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SPT5 antibody
See all SPT5 primary antibodies
DescriptionRabbit polyclonal to SPT5
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Orangutan
Synthetic peptide within Human SPT5 aa 50-100. The exact sequence is proprietary. NP_003160.2
Database link: O00267
- IP: HeLa whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245650 was affinity purified using an epitope specific to SPT5 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
FunctionComponent of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the SPT5 family.
Contains 5 KOW domains.
modificationsMethylated by PRMT1/HRMT1L2 and PRMT5/SKB1. Methylation negatively regulates interaction with P-TEFb and RNA polymerase II.
Phosphorylated. Phosphorylation by P-TEFb alleviates transcriptional pausing and can stimulate transcriptional elongation from the HIV-1 LTR. P-TEFb dependent phosphorylation is stimulated by the HIV-1 Tat protein. Phosphorylation may also stimulate interaction with PIN1. Bulk phosphorylation occurs predominantly in mitosis.
- Information by UniProt
- DRB sensitivity inducing factor large subunit antibody
- DRB sensitivity-inducing factor 160 kDa subunit antibody
- DRB sensitivity-inducing factor large subunit antibody
SPT5 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245650 used for IP at 3 µg/mg lysate. For WB another anti-SPT5 antibody was used at 1 µg/ml.
Lane 1: ab245650 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Chemiluminescence detection: 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245650 has not yet been referenced specifically in any publications.