Overview

  • Product name

  • Description

    Rabbit polyclonal to SPT5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Orangutan
  • Immunogen

    Synthetic peptide within Human SPT5 aa 50-100. The exact sequence is proprietary. NP_003160.2
    Database link: O00267

  • Positive control

    • IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245650 was affinity purified using an epitope specific to SPT5 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
    • Tissue specificity

      Ubiquitously expressed.
    • Sequence similarities

      Belongs to the SPT5 family.
      Contains 5 KOW domains.
    • Post-translational
      modifications

      Methylated by PRMT1/HRMT1L2 and PRMT5/SKB1. Methylation negatively regulates interaction with P-TEFb and RNA polymerase II.
      Phosphorylated. Phosphorylation by P-TEFb alleviates transcriptional pausing and can stimulate transcriptional elongation from the HIV-1 LTR. P-TEFb dependent phosphorylation is stimulated by the HIV-1 Tat protein. Phosphorylation may also stimulate interaction with PIN1. Bulk phosphorylation occurs predominantly in mitosis.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • DRB sensitivity inducing factor large subunit antibody
      • DRB sensitivity-inducing factor 160 kDa subunit antibody
      • DRB sensitivity-inducing factor large subunit antibody
      • DSIF large subunit antibody
      • DSIF p160 antibody
      • FLJ34157 antibody
      • hSPT 5 antibody
      • hSPT5 antibody
      • SPT 5 antibody
      • SPT 5H antibody
      • SPT5 antibody
      • SPT5H antibody
      • SPT5H_HUMAN antibody
      • Suppressor of Ty 5 homolog antibody
      • Suppressor of Ty 5 homolog (S. cerevisiae) antibody
      • SUPT 5H antibody
      • supt5h antibody
      • Tat cotransactivator 1 protein antibody
      • Tat CT1 antibody
      • Tat CT1 protein antibody
      • Tat-cotransactivator 1 protein antibody
      • Tat-CT1 protein antibody
      • Transcription elongation factor SPT 5 antibody
      • Transcription elongation factor spt5 antibody
      • Transcription factor Tat CT1 antibody
      see all

    Images

    • SPT5 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

      ab245650 used for IP at 3 µg/mg lysate. For WB another anti-SPT5 antibody was used at 1 µg/ml.

      Lane 1: ab245650 IP in HeLa whole cell lysate.
      Lane 2: Control IgG in HeLa whole cell lysate.

      Chemiluminescence detection: 30 seconds.

    References

    ab245650 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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