• Product name

    Anti-SPT5 antibody [EPR5145(2)]
    See all SPT5 primary antibodies
  • Description

    Rabbit monoclonal [EPR5145(2)] to SPT5
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SPT5 aa 750-850. The exact sequence is proprietary.

  • Positive control

    • HeLa, HepG2, RAW264.7, PC12, NIH3T3, Jurkat and 293T cell lysates; Human colon tissue; HeLa cells.
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 5.31 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

  • Clone number

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab126592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 121 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
    • Tissue specificity

      Ubiquitously expressed.
    • Sequence similarities

      Belongs to the SPT5 family.
      Contains 5 KOW domains.
    • Post-translational

      Methylated by PRMT1/HRMT1L2 and PRMT5/SKB1. Methylation negatively regulates interaction with P-TEFb and RNA polymerase II.
      Phosphorylated. Phosphorylation by P-TEFb alleviates transcriptional pausing and can stimulate transcriptional elongation from the HIV-1 LTR. P-TEFb dependent phosphorylation is stimulated by the HIV-1 Tat protein. Phosphorylation may also stimulate interaction with PIN1. Bulk phosphorylation occurs predominantly in mitosis.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • DRB sensitivity inducing factor large subunit antibody
      • DRB sensitivity-inducing factor 160 kDa subunit antibody
      • DRB sensitivity-inducing factor large subunit antibody
      • DSIF large subunit antibody
      • DSIF p160 antibody
      • FLJ34157 antibody
      • hSPT 5 antibody
      • hSPT5 antibody
      • SPT 5 antibody
      • SPT 5H antibody
      • SPT5 antibody
      • SPT5H antibody
      • SPT5H_HUMAN antibody
      • Suppressor of Ty 5 homolog antibody
      • Suppressor of Ty 5 homolog (S. cerevisiae) antibody
      • SUPT 5H antibody
      • supt5h antibody
      • Tat cotransactivator 1 protein antibody
      • Tat CT1 antibody
      • Tat CT1 protein antibody
      • Tat-cotransactivator 1 protein antibody
      • Tat-CT1 protein antibody
      • Transcription elongation factor SPT 5 antibody
      • Transcription elongation factor spt5 antibody
      • Transcription factor Tat CT1 antibody
      see all


    • All lanes : Anti-SPT5 antibody [EPR5145(2)] (ab126592) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : RAW264.7 cell lysate
      Lane 4 : PC12 cell lysate
      Lane 5 : NIH 3T3 cell lysate
      Lane 6 : Jurkat cell lysate
      Lane 7 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 121 kDa

    • ab126592 at 1/100 dilution staining SPT5 in paraffin-embedded Human colon tissue by immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab126592 at 1/100 dilution staining SPT5 in HeLa cells by immunofluorescence.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    This product has been referenced in:

    • Methot SP  et al. A licensing step links AID to transcription elongation for mutagenesis in B cells. Nat Commun 9:1248 (2018). Mouse . Read more (PubMed: 29593215) »
    • Bolt MJ  et al. Systems level-based RNAi screening by high content analysis identifies UBR5 as a regulator of estrogen receptor-a protein levels and activity. Oncogene N/A:N/A (2014). Human . Read more (PubMed: 24441042) »
    See all 2 Publications for this product

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