• Product name
  • Description
    Rabbit polyclonal to Spt6
  • Host species
  • Tested applications
    Suitable for: IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1150 - 1250 of Human Spt6.

    Read Abcam's proprietary immunogen policy (Peptide available as ab32819.)

  • Positive control
    • This antibody gave a positive signal for the following cell lysates: HeLa Nuclear; HeLa; Jurkat.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab32820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/30. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
WB 1/500. Detects a band of approximately 230 kDa (predicted molecular weight: 199 kDa).



  • Lane 1 : Marker
    Lanes 2-4 : Anti-Spt6 antibody (ab32820) at 1/500 dilution
    Lane 5 : High Mark marker

    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 3 : Jurkat whole cell lysate (ab7899) at 20 µg
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg

    Lanes 2-4 : IR Dye 680 Conjugated Goat anti Rabbit IgG (H+L) at 1/21250 dilution

    Performed under reducing conditions.

    Predicted band size: 199 kDa
    Observed band size: 230 kDa
    why is the actual band size different from the predicted?

    Ab32820 detects a band at approximately 230kDa.  Other antibodies to this target detect a band in this region and it is understood that this band corresponds to the native Spt6 protein.
  • Image courtesy of Human Protein Atlas

    ab32820 staining Spt6 in human gall bladder, showing a distinct and strong staining pattern in glandular cells. Paraffin embedded human skin tissue was incubated with ab32820 (1/30 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab32820 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

  • Spt6 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Spt6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32820.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 230kDa: Spt6


This product has been referenced in:
  • Soboleva TA  et al. A new link between transcriptional initiation and pre-mRNA splicing: The RNA binding histone variant H2A.B. PLoS Genet 13:e1006633 (2017). ChIP ; Mouse . Read more (PubMed: 28234895) »
  • Dembowski JA  et al. Replication-Coupled Recruitment of Viral and Cellular Factors to Herpes Simplex Virus Type 1 Replication Forks for the Maintenance and Expression of Viral Genomes. PLoS Pathog 13:e1006166 (2017). Read more (PubMed: 28095497) »
See all 9 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Human Cell lysate - nuclear (HeLa)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C

Dr. Paul Lesbats

Verified customer

Submitted Aug 30 2013


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