Overview

  • Product name

  • Description

    Rabbit polyclonal to SPTBN1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human SPTBN1 (internal sequence). The exact sequence is proprietary.
    Database link: Q01082

  • Positive control

    • IHC-P: Rat pancreas tissue. WB: Mouse and rat brain tissue extracts. HEK-293T, A431, HeLa and HepG2 whole cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab229478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/10000. Predicted molecular weight: 275 kDa.

Target

  • Function

    Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
  • Tissue specificity

    Isoform 2 is present in brain, lung and kidney (at protein level).
  • Sequence similarities

    Belongs to the spectrin family.
    Contains 2 CH (calponin-homology) domains.
    Contains 1 PH domain.
    Contains 17 spectrin repeats.
  • Post-translational
    modifications

    Isoform 2 is phosphorylated on Ser-8 and Ser-10.
  • Cellular localization

    Cell membrane and Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > M line. Colocalizes with ANK2 in a distinct intracellular compartment of neonatal cardiomyocytes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta G spectrin antibody
    • Beta II spectrin antibody
    • Beta spectrin 2 antibody
    • Beta spectrin II antibody
    • Beta-II spectrin antibody
    • betaSpII antibody
    • brain 1 antibody
    • ELF antibody
    • Embryonic liver beta fodrin antibody
    • Fodrin beta chain antibody
    • non-erythroid beta chain 1 antibody
    • Spectrin antibody
    • Spectrin beta chain antibody
    • Spectrin, beta, non erythrocytic 1 antibody
    • Spectrin, non erythroid beta chain 1 antibody
    • SPTB2 antibody
    • SPTB2_HUMAN antibody
    • SPTBN1 antibody
    see all

Images

  • Paraffin-embedded rat pancreas tissue stained for SPTBN1 with ab229478 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-SPTBN1 antibody (ab229478) at 1/1000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
    Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell extracts
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 275 kDa



    5% SDS-PAGE

  • All lanes : Anti-SPTBN1 antibody (ab229478) at 1/500 dilution

    Lane 1 : Mouse brain tissue extracts
    Lane 2 : Rat brain tissue extracts

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 275 kDa



    5% SDS-PAGE

References

ab229478 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229478.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up