Key features and details
- Rabbit polyclonal to SPTBN1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SPTBN1 antibody
See all SPTBN1 primary antibodies
DescriptionRabbit polyclonal to SPTBN1
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Horse, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
Synthetic peptide from a region between residue 2100 and 2150 of human SPTBN1 (NP_003119.2)
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 274 kDa (predicted molecular weight: 274 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionFodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Tissue specificityIsoform 2 is present in brain, lung and kidney (at protein level).
Sequence similaritiesBelongs to the spectrin family.
Contains 2 CH (calponin-homology) domains.
Contains 1 PH domain.
Contains 17 spectrin repeats.
modificationsIsoform 2 is phosphorylated on Ser-8 and Ser-10.
Cellular localizationCell membrane and Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > M line. Colocalizes with ANK2 in a distinct intracellular compartment of neonatal cardiomyocytes.
- Information by UniProt
- Beta G spectrin antibody
- Beta II spectrin antibody
- Beta spectrin 2 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling SPTBN1 with ab72239 at 1/1000 (0.2µg/ml). Detection: DAB.
All lanes : Anti-SPTBN1 antibody (ab72239) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 274 kDa
Observed band size: 274 kDa
Immunoprecipitation/ Western Blot of SPTBN1.
Lane 1: ab72239 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
ab72239 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 1 second.
IHC image of ab72239 staining in human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72239, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab72239 has been referenced in 5 publications.
- Liang L et al. Validation of a multi-omics strategy for prioritizing personalized candidate driver genes Oncotarget 7:38440-38450 (2016). PubMed: 27469031
- Ji F et al. The prognostic value of combined TGF-ß1 and ELF in hepatocellular carcinoma. BMC Cancer 15:116 (2015). IHC . PubMed: 25880619
- Kiriyama S et al. CEACAM1 long cytoplasmic domain isoform is associated with invasion and recurrence of hepatocellular carcinoma. Ann Surg Oncol 21 Suppl 4:S505-14 (2014). PubMed: 24390710
- Forster F et al. Guanylate binding protein 1-mediated interaction of T cell antigen receptor signaling with the cytoskeleton. J Immunol 192:771-81 (2014). PubMed: 24337748
- Dai L et al. IL-27 inhibits HIV-1 infection in human macrophages by down-regulating host factor SPTBN1 during monocyte to macrophage differentiation. J Exp Med 210:517-34 (2013). PubMed: 23460728