Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5869] to SPTBN1
- Suitable for: WB, IHC-P, ICC
- Reacts with: Mouse, Rat, Human
Product nameAnti-SPTBN1 antibody [EPR5869]
See all SPTBN1 primary antibodies
DescriptionRabbit monoclonal [EPR5869] to SPTBN1
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human SPTBN1 aa 1900-2000. The exact sequence is proprietary.
- Human kidney tissue; SH-SY5Y, HeLa, Caco 2 and Jurkat whole cell lysate (ab7899).
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 4.40 x 10 -11 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 270 kDa (predicted molecular weight: 275 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)|
|ICC||1/100 - 1/250.|
FunctionFodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Tissue specificityIsoform 2 is present in brain, lung and kidney (at protein level).
Sequence similaritiesBelongs to the spectrin family.
Contains 2 CH (calponin-homology) domains.
Contains 1 PH domain.
Contains 17 spectrin repeats.
modificationsIsoform 2 is phosphorylated on Ser-8 and Ser-10.
Cellular localizationCell membrane and Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > M line. Colocalizes with ANK2 in a distinct intracellular compartment of neonatal cardiomyocytes.
- Information by UniProt
- Beta G spectrin antibody
- Beta II spectrin antibody
- Beta spectrin 2 antibody
ab124888, at 1/250 dilution, staining SPTBN1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
All lanes : Anti-SPTBN1 antibody [EPR5869] (ab124888) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Caco 2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 275 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124888 has not yet been referenced specifically in any publications.