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Rabbit monoclonal [EPR5869] to SPTBN1
, ICCmore detailsUnsuitable for:
Flow Cyt or IP
Mouse, Rat, Human
Synthetic peptide within Human SPTBN1 aa 1900-2000. The exact sequence is proprietary.
- Human kidney tissue; SH-SY5Y, HeLa, Caco 2 and Jurkat cell lysates.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000 - 1/10000. Detects a band of approximately 270 kDa (predicted molecular weight: 275 kDa).
||1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
||1/100 - 1/250.
Is unsuitable for Flow Cyt or IP.
Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Isoform 2 is present in brain, lung and kidney (at protein level).
Belongs to the spectrin family.
Contains 2 CH (calponin-homology) domains.
Contains 1 PH domain.
Contains 17 spectrin repeats.
Isoform 2 is phosphorylated on Ser-8 and Ser-10.
Cell membrane and Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > M line. Colocalizes with ANK2 in a distinct intracellular compartment of neonatal cardiomyocytes.
Information by UniProt
- Beta G spectrin antibody
- Beta II spectrin antibody
- Beta spectrin 2 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTBN1 antibody [EPR5869] (ab124888)
ab124888, at 1/250 dilution, staining SPTBN1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Western blot - Anti-SPTBN1 antibody [EPR5869] (ab124888)
All lanes : Anti-SPTBN1 antibody [EPR5869] (ab124888) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Caco 2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 275 kDa
Other - Anti-SPTBN1 antibody [EPR5869] (ab124888)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"