Overview

  • Product name

    Anti-SPTBN2 antibody [ABM5A16]
    See all SPTBN2 primary antibodies
  • Description

    Mouse monoclonal [ABM5A16] to SPTBN2
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human SPTBN2 aa 1280-1450.
    Database link: O15020

  • Positive control

    • WB: Human brain and kidney lysates. Flow Cyt: A431 and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2-4µg for 106 cells.
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 271, 268 kDa.

Target

  • Relevance

    SPTBN2 (Spectrin beta non erythrocytic 2) probably plays an important role in neuronal membrane skeleton. It is highly expressed in Purkinje cells and stabilizes the glutamate transporter EAAT4 at the surface of the plasma membrane.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • Beta III spectrin antibody
    • Glutamate transporter EAAT4 associated protein 41 antibody
    • GTRAP41 antibody
    • SCA 5 antibody
    • SCA5 antibody
    • SCAR14 antibody
    • Spectrin beta chain brain 2 antibody
    • Spectrin beta III sigma 2 antibody
    • Spectrin beta non erythrocytic 2 antibody
    • Spectrin non erythroid beta chain 2 antibody
    • Spinocerebellar ataxia 5 antibody
    • Spinocerebellar ataxia 5 protein antibody
    • SPTBN 2 antibody
    see all

Images

  • All lanes : Anti-SPTBN2 antibody [ABM5A16] (ab228447) at 0.1 µg/ml

    Lane 1 : Human kidney lysate
    Lane 2 : Human brain lysate

    Predicted band size: 271, 268 kDa

  • Flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cells, labeling SPTBN2 with ab228447 at 2 µg/106 cells (red) compared to an isotype control (green). Goat anti-mouse PE conjugate was used as secondary antibody.

    Cells were fixed with 4% paraformaldehyde for 10 minutes and washed with PBS by centrifuging at 1100 for 5 minutes followed by permeabilization for 20 minutes and washed again. The cells were incubated with primary antibody for 45 minutes and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 minutes. Data acquisition was done after washing twice with PBS.

     

     

  • Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling SPTBN2 with ab228447 at 2 µg/106 cells (red) compared to an isotype control (green). Goat anti-mouse PE conjugate was used as secondary antibody.

    Cells were fixed with 4% paraformaldehyde for 10 minutes and washed with PBS by centrifuging at 1100 for 5 minutes followed by permeabilization for 20 minutes and washed again. The cells were incubated with primary antibody for 45 minutes and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 minutes. Data acquisition was done after washing twice with PBS.

     

     

References

ab228447 has not yet been referenced specifically in any publications.

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