Key features and details
- Rabbit polyclonal to SPTLC1
- Suitable for: IHC-P, WB
- Isotype: IgG
Product nameAnti-SPTLC1 antibody
See all SPTLC1 primary antibodies
DescriptionRabbit polyclonal to SPTLC1
Tested applicationsSuitable for: IHC-P, WBmore details
Recombinant fragment (His-T7-tag) corresponding to Mouse SPTLC1 aa 143-473. Two N-terminal tags (Expressed in E.coli).
DVHLDLEERLAKFMKTEEAIIYSYGFSTIASAIPAYSKRGDIIFVDSAAC FAIQKGLQASRSDIKLFKHNDVADLERLLKEQEIEDQKNPRKARVTRRFI VVEGLYMNTGTICPLPELVKLKYKYKARIFLEESLSFGVLGEHGRGVTEH YGISIDDIDLISANMENALASVGGFCCGRSFVVDHQRLSGQGYCFSASLP PLLAAAAIEALNIMEENPDIFAVLKKKCQNIHKSLQGVSGLKVVGESLSP ALHLQLEESTGSREKDVKLLQAIVDQCMDKGIALTQARYLDKEEKCLPPP SIRVVVTVEQTEEELQRAASTIREAAQAVLL
Database link: O35704
- IHC-P: Mouse kidney, uterus and liver tissue. WB: Mouse liver, lung and brain tissue; Recombinant mouse SPTLC1 protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab232847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml.|
FunctionSerine palmitoyltransferase (SPT). The heterodimer formed with LCB2 (SPTLC2 or SPTLC3) constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SSSPTA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SSSPTA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SSSPTB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SSSPTB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference.
Tissue specificityWidely expressed. Not detected in small intestine.
PathwayLipid metabolism; sphingolipid metabolism.
Involvement in diseaseDefects in SPTLC1 are the cause of hereditary sensory and autonomic neuropathy type 1A (HSAN1A) [MIM:162400]. The hereditary sensory and autonomic neuropathies are a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN1A is an autosomal dominant axonal neuropathy with onset in the second or third decades. Initial symptoms are loss of pain, touch, heat, and cold sensation over the feet, followed by distal muscle wasting and weakness. Loss of pain sensation leads to chronic skin ulcers and distal amputations.
Sequence similaritiesBelongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- HSAN antibody
- HSAN1 antibody
- HSN1 antibody
Formalin-fixed, paraffin-embedded mouse uterus tissue stained for SPTLC1 using ab232847 at 20 µg/mL in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded mouse kidney tissue stained for SPTLC1 using ab232847 at 20 µg/mL in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded mouse liver tissue stained for SPTLC1 using ab232847 at 20 µg/mL in immunohistochemical analysis. DAB staining.
All lanes : Anti-SPTLC1 antibody (ab232847) at 1 µg/ml
Lane 1 : Mouse liver lysate
Lane 2 : Mouse lung lysate
Lane 3 : Mouse brain lysate
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Anti-SPTLC1 antibody (ab232847) at 1 µg/ml + Recombinant mouse SPTLC1 protein
HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232847 has not yet been referenced specifically in any publications.