Key features and details
- Rabbit polyclonal to SPTLC3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SPTLC3 antibody
See all SPTLC3 primary antibodies
DescriptionRabbit polyclonal to SPTLC3
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment (His-T7-tag) corresponding to Mouse SPTLC3 aa 80-563. Expressed in E. coli. N-terminal tags.
RDFMRNWGIEKCNAAVEREEQKDFVPLYQDFENFYKRNLYMRIRDSWSHT VCSAPEPYMNVMEKVTDDYNWTFRHTGKVIENIINMASYNYLGLAGKYDD SMVRVKDTLEKYGVGVASTRNEMGTLDIHKELEDLMAEFLNVEAVMSFGM GFATNAMNIPVFVGKGCLILSDEFNHTSVILGSRLSGAVIRPFKHNNAEN LEKLLREAIIRGQPGTGRAWKKILIVVEGVYSMEGSIVNLAQIVALKKKY KAYLYIDEAHSIGCTGPTGRGVRELFGLDPEDIDVYMGTFTKSFSGSGGY IGGKKEIVDYLRMQSHSTTYATSMSPVVAAQLIRSLKITMGYEGNIGGME RIQQLKENIKYFRRRLKEMGFIIYGNDFSPVIPVLLYMPAKVSAFSRFLL KKKISVVVVGFPATSLPEGRARFSMSSAHTREMLDTVLEVVDELGDLLNV KYFPLKKSGRAILYNKEGFDNEASFEEMHSEPEA
Database link: Q8BG54
- WB: Recombinant mouse SPTLC3 protein; HeLa and HEK-293T cell lysates. IHC-P: Mouse kidney tissue.
This product was previously labelled as Serine palmitoyltransferase 3
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 63 kDa.|
FunctionSerine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC3-SSSPTA isozyme uses both C14-CoA and C16-CoA as substrates, while the SPTLC1-SPTLC3-SSSPTB has the ability to use a broader range of acyl-CoAs without apparent preference.
Tissue specificityExpressed in most tissues, except peripheral blood cells and bone marrow, with highest levels in heart, kidney, liver, uterus and skin.
PathwayLipid metabolism; sphingolipid metabolism.
Sequence similaritiesBelongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- LCB 3 antibody
- LCB2b antibody
- Long chain base biosynthesis protein 2b antibody
Formalin-fixed, paraffin-embedded mouse kidney tissue stained for SPTLC3 with ab232829 at 20 µg/ml in immunohistochemical analysis. DAB staining.
All lanes : Anti-SPTLC3 antibody (ab232829) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
All lanes : HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 63 kDa
Anti-SPTLC3 antibody (ab232829) at 2 µg/ml + Recombinant mouse SPTLC3 protein
Predicted band size: 63 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232829 has not yet been referenced specifically in any publications.