Anti-SPTLC3 antibody (ab237532)
Key features and details
- Rabbit polyclonal to SPTLC3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SPTLC3 antibody
See all SPTLC3 primary antibodies -
Description
Rabbit polyclonal to SPTLC3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human SPTLC3 aa 1-100.
Database link: Q9NUV7 -
Positive control
- WB: Mouse liver and kidney lysates; Rat heart and kidney lysates; U-87 MG, HepG2, HEK-293T and A549 whole cell lysates. IHC-P: Human liver cancer tissue. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity greater than 95% -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 62 kDa.
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IHC-P |
1/200 - 1/500.
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 62 kDa. |
IHC-P
1/200 - 1/500. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC3-SSSPTA isozyme uses both C14-CoA and C16-CoA as substrates, while the SPTLC1-SPTLC3-SSSPTB has the ability to use a broader range of acyl-CoAs without apparent preference. -
Tissue specificity
Expressed in most tissues, except peripheral blood cells and bone marrow, with highest levels in heart, kidney, liver, uterus and skin. -
Pathway
Lipid metabolism; sphingolipid metabolism. -
Sequence similarities
Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 55304 Human
- Entrez Gene: 228677 Mouse
- Entrez Gene: 296188 Rat
- Omim: 611120 Human
- SwissProt: Q9NUV7 Human
- SwissProt: Q8BG54 Mouse
- Unigene: 425023 Human
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Alternative names
- LCB 3 antibody
- LCB2b antibody
- Long chain base biosynthesis protein 2b antibody
see all
Images
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All lanes : Anti-SPTLC3 antibody (ab237532) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 62 kDa -
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining human colon cancer using ab237532 at 1/100.
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Paraffin-embedded human liver cancer tissue stained for SPTLC3 using ab237532 at 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
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HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for SPTLC3 (green) using ab237532 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
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All lanes : Anti-SPTLC3 antibody (ab237532) at 1/1000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 4 : Rat heart tissue lysate
Lane 5 : Rat kidney tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 62 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab237532 has not yet been referenced specifically in any publications.