Key features and details
- Rabbit polyclonal to SPTY2D1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab150851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
RelevanceFunction: Histone chaperone that stabilizes pre-existing histone tetramers and regulates replication-independent histone exchange on chromatin (PubMed:26109053). Required for normal chromatin refolding in the coding region of transcribed genes, and for the suppression of spurious transcription (PubMed:26109053). Binds DNA and histones and promotes nucleosome assembly (in vitro) (PubMed:23378026, PubMed:26109053). Facilitates formation of tetrameric histone complexes containing histone H3 and H4 (PubMed:26109053). Modulates RNA polymerase 1-mediated transcription (By similarity). Binds DNA, with a preference for branched DNA species, such as Y-form DNA and Holliday junction DNA (PubMed:23378026). Similarity: Belongs to the SPT2 family. Domain: The acidic C-terminal domain mediates interaction with histone H3/H4 complexes.
- DKFZp686I068 antibody
- FLJ39441 antibody
- Protein KU002155 antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleoli and cytoplasm. Recommended concentration of ab150851 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemical analysis of Paraffin-embedded Human breast tissue, labeling SPTY2D1 with ab150851 at 1/50 dilution.
ab150851 has not yet been referenced specifically in any publications.