Overview

  • Product name

  • Description

    Rabbit polyclonal to SPTY2D1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 164-251 of Human SPTY2D1 (Q68D10).

  • Positive control

    • Human breast tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Relevance

    Function: Histone chaperone that stabilizes pre-existing histone tetramers and regulates replication-independent histone exchange on chromatin (PubMed:26109053). Required for normal chromatin refolding in the coding region of transcribed genes, and for the suppression of spurious transcription (PubMed:26109053). Binds DNA and histones and promotes nucleosome assembly (in vitro) (PubMed:23378026, PubMed:26109053). Facilitates formation of tetrameric histone complexes containing histone H3 and H4 (PubMed:26109053). Modulates RNA polymerase 1-mediated transcription (By similarity). Binds DNA, with a preference for branched DNA species, such as Y-form DNA and Holliday junction DNA (PubMed:23378026). Similarity: Belongs to the SPT2 family. Domain: The acidic C-terminal domain mediates interaction with histone H3/H4 complexes.
  • Database links

  • Alternative names

    • DKFZp686I068 antibody
    • FLJ39441 antibody
    • Protein KU002155 antibody
    • Protein SPT2 homolog antibody
    • SPT2 domain-containing protein 1 antibody
    • SPT2, Suppressor of Ty, domain containing 1 (S. cerevisiae) antibody
    • SPT2_HUMAN antibody
    • Spty2d1 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleoli and cytoplasm. Recommended concentration of ab150851 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemical analysis of Paraffin-embedded Human breast tissue, labeling SPTY2D1 with ab150851 at 1/50 dilution.

References

ab150851 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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