Overview

  • Product name
  • Description
    Rabbit polyclonal to SPTY2D1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Sheep, Rabbit, Horse, Cow, Dog, Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human SPTY2D1 aa 75-125 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary. from Isoform 3.
    Database link: Q68D10.3

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab215722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 76 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance
      Function: Histone chaperone that stabilizes pre-existing histone tetramers and regulates replication-independent histone exchange on chromatin (PubMed:26109053). Required for normal chromatin refolding in the coding region of transcribed genes, and for the suppression of spurious transcription (PubMed:26109053). Binds DNA and histones and promotes nucleosome assembly (in vitro) (PubMed:23378026, PubMed:26109053). Facilitates formation of tetrameric histone complexes containing histone H3 and H4 (PubMed:26109053). Modulates RNA polymerase 1-mediated transcription (By similarity). Binds DNA, with a preference for branched DNA species, such as Y-form DNA and Holliday junction DNA (PubMed:23378026). Similarity: Belongs to the SPT2 family. Domain: The acidic C-terminal domain mediates interaction with histone H3/H4 complexes.
    • Database links
    • Alternative names
      • DKFZp686I068 antibody
      • FLJ39441 antibody
      • Protein KU002155 antibody
      • Protein SPT2 homolog antibody
      • SPT2 domain-containing protein 1 antibody
      • SPT2, Suppressor of Ty, domain containing 1 (S. cerevisiae) antibody
      • SPT2_HUMAN antibody
      • Spty2d1 antibody
      see all

    Images

    • All lanes : Anti-SPTY2D1 antibody (ab215722) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate
      Lane 2 : 293T whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 76 kDa


      Exposure time: 3 minutes

    References

    ab215722 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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