Western blot abreview for Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Buffer
Dilution
1/20000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-Rabbit Ig
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ELC + X-ray
Exposure
30 second(s)
Bands
Specific: ~62 kDa
Positive control
N2A cell lysate

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Submitted Jun 20 2017

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