Western blot abreview for Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

Excellent
Abreviews
Application
Western blot
Sample
Cow Cell lysate - whole cell (Kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Buffer
Dilution
1/20000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-Rabbit Ig
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ELC + X-ray
Exposure
30 second(s)
Bands
Specific: ~62 kDa
Positive control
N2A cell lysate

Additional data

Abcam response
Thank you for your valuable feedback about the use of ab109012 in BOAC cells. We have not tested the antibody in cow and appreciate the time and effort that the reviewer has put in to testing this antibody in this species. The homology of the immunogen and bovine p62 is 100% and the data show that ab109012 is cross-reacting with the bovine protein. We welcome any further feedback and AbReviews from other scientist who have used this antibody in this species.

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Verified customer

Submitted Jun 20 2017

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