Immunocytochemistry abreview for Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

Excellent
Abreviews
Application
Immunocytochemistry
Sample
Human Cell (Hela cells (Epithelial))
Permeabilization
Yes - 0.2% Triron X100
Specification
Hela cells (Epithelial)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Methanol

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Buffer
Dilution
1/2000

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: Anti-Rabbit Ig
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 568

Additional data

Additional Notes
The autophagy was inducted by EBSS then lysosomal degradation was inhibited by bafilomycine a1. The figure showing p62 vesicles.

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Submitted Aug 14 2017

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