Immunoprecipitation abreview for Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

Excellent
Abreviews
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (MEF cells)
Total protein in input
500 µg
Immuno-precipitation step
Protein A/G
Specification
MEF cells

Other product details

Incubation time
3 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Lysys buffer
Concentration
10 µl/mg lysate

Western Blot antibody

Dilution
1/1000

Additional data

Additional Notes
500 ug of whole cell lysates from MEF cells were incubated with 5 ul of antibody for 3 hours at 4 degree. Then, protein A/G magnetic beads were added and incubated at 4 degree for 2 hours.

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Submitted Nov 30 2018

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