Anti-SQSTM1 / p62 antibody (ab155686)

Rabbit polyclonal SQSTM1 / p62 antibody. Validated in WB, IP, IHC, Flow Cyt, ICC/IF and tested in Mouse, Rat, Human, Zebrafish. Cited in 7 publication(s). Independently reviewed in 3 review(s).


  • Product name
    Anti-SQSTM1 / p62 antibody
    See all SQSTM1 / p62 primary antibodies
  • Description
    Rabbit polyclonal to SQSTM1 / p62
  • Host species
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human SQSTM1/ p62 aa 157-251 (internal sequence).

  • Positive control
    • A549 whole cell lysate; Huh7 whole cell lysate, untreated and teated with 3µM-Thapsipargin for 12hrs; HeLa cells.



Our Abpromise guarantee covers the use of ab155686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
Flow Cyt 1/50 - 1/200.
IP 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
ICC/IF 1/100 - 1/1000.


  • Function
    Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.
  • Tissue specificity
    Ubiquitously expressed.
  • Involvement in disease
    Defects in SQSTM1 are a cause of Paget disease of bone (PDB) [MIM:602080]. PDB is a metabolic bone disease affecting the axial skeleton and characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Manifestations of the disease include bone pain, deformity, pathological fractures, deafness, neurological complications and increased risk of osteosarcoma. PDB is a chronic disease affecting 2 to 3% of the population above the age of 40 years.
  • Sequence similarities
    Contains 1 OPR domain.
    Contains 1 UBA domain.
    Contains 1 ZZ-type zinc finger.
  • Domain
    The UBA domain binds specifically 'Lys-63'-linked polyubiquitin chains of polyubiquitinated substrates. Mediates the interaction with TRIM55.
    The OPR domain mediates homooligomerization and interactions with PRKCZ, PRKCI, MAP2K5 and NBR1.
    The ZZ-type zinc finger mediates the interaction with RIPK1.
  • Post-translational
    Phosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN.
  • Cellular localization
    Cytoplasm. Late endosome. Nucleus. Sarcomere (By similarity). In cardiac muscles localizes to the sarcomeric band (By similarity). Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma.
  • Information by UniProt
  • Database links
  • Alternative names
    • A170 antibody
    • DMRV antibody
    • EBI 3 associated protein of 60 kDa antibody
    • EBI 3 associated protein p60 antibody
    • EBI3 associated protein of 60 kDa antibody
    • EBI3 associated protein p60 antibody
    • EBI3-associated protein of 60 kDa antibody
    • EBIAP antibody
    • FTDALS3 antibody
    • MGC127197 antibody
    • ORCA antibody
    • OSF-6 antibody
    • Osi antibody
    • OSIL antibody
    • Oxidative stress induced like antibody
    • p60 antibody
    • p62 antibody
    • p62B antibody
    • Paget disease of bone 3 antibody
    • PDB 3 antibody
    • PDB3 antibody
    • Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa antibody
    • Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody
    • Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody
    • PKC-zeta-interacting protein antibody
    • Protein kinase C-zeta-interacting protein antibody
    • Sequestosome 1 antibody
    • Sequestosome-1 antibody
    • SQSTM 1 antibody
    • SQSTM_HUMAN antibody
    • Sqstm1 antibody
    • STAP antibody
    • STONE14 antibody
    • Ubiquitin binding protein p62 antibody
    • Ubiquitin-binding protein p62 antibody
    • ZIP 3 antibody
    • ZIP antibody
    • ZIP3 antibody
    see all


  • All lanes : Anti-SQSTM1 / p62 antibody (ab155686) at 1/1000 dilution

    Lane 1 : PC-12 whole cell lysate/extract
    Lane 2 : Rat2 whole cell lysate/extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 47 kDa

    10% SDS-PAGE

  • Immunocytochemistry/Immunofluorescent analysis of HeLa mock (left) and treated with 50μM Chloroquine for 24 hr (right) labeling SQSTM1/p62 (green) with ab155686 at 1/1000 dilution. Cells were fixed with 4% paraformaldehyde at RT for 15 min. Red: Phalloidin, a F-actin marker.

  • Ab155686 at 1/500 immunoprecipitating SQSTM1 / p62 in HepG2 whole cell extracts.

    Lane (-):  Non- transfected HepG2 whole cell extracts.
    Lane (+): Transfected HepG2 whole cell extracts and membrane blotted with ab155686.

    For Western blotting, HRP conjugated anti- rabbit IgG was used as secondary antibody.

  • Paraffin-embedded human ovarian carcinoma tissue stained for SQSTM1 / p62 with ab155686 (1/500) in Immunohistochemical analysis.

  • 4% paraformaldehyde-fixed HeLa cells stained for SQSTM1 / p62 (blue) using ab155686 (1/500) in Flow Cytometry.

  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling SQSTM1 / p62 with ab155686 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Anti-SQSTM1 / p62 antibody (ab155686) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size: 47 kDa

    10% SDS PAGE
  • All lanes : Anti-SQSTM1 / p62 antibody (ab155686) at 1/1500 dilution

    Lane 1 : Huh7 whole cell
    lysate, untreated
    Lane 2 : Huh7 whole cell
    lysate, treated with 3µM-Thapsipargin
    for 12hrs

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 47 kDa

    10% SDS PAGE


This product has been referenced in:
  • Gong G  et al. Tanshinone IIA alleviates oxidative damage after spinal cord injury in vitro and in vivo through up-regulating miR-124. Life Sci 216:147-155 (2019). Read more (PubMed: 30468834) »
  • Ma Z  et al. Autophagy promotes hepatic differentiation of hepatic progenitor cells by regulating the Wnt/ß-catenin signaling pathway. J Mol Histol 50:75-90 (2019). Read more (PubMed: 30604254) »
See all 9 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Frozen sections)
Zebrafish Tissue sections (hindbrain at 8 somites)
Yes - triton x-100 0.1%
hindbrain at 8 somites
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Adrian Santos

Verified customer

Submitted Nov 15 2018

Western blot
Human Cell lysate - whole cell (293T)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 22 2018

Mouse Cell lysate - whole cell (BV2)
Total protein in input
100 µg
10 nM for 24hrs
Immuno-precipitation step
Protein A/G

Dr. jianhai long

Verified customer

Submitted Jan 15 2018


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