Product nameAnti-SQSTM1 / p62 antibody
See all SQSTM1 / p62 primary antibodies
DescriptionRabbit polyclonal to SQSTM1 / p62
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IF, IHC-FoFrmore details
Species reactivityReacts with: Mouse, Rat, Human
A 14 amino acid peptide from near the Carboxy terminus of Human SQSTM1/ p62 (GenBank accession no. Q13501).
- WB: Mouse gastrocnemius lysate; Human spleen tissue lysate. IHC: Mouse VCPR155H/R155H quadricep tissue. IHC-P: Human spleen tissue. ICC/IF: HeLa cells; Murine embryonic fibroblast; Rat spleen cells.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab91526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 47 kDa.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 21757726|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 22898872|
FunctionAdapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.
Tissue specificityUbiquitously expressed.
Involvement in diseaseDefects in SQSTM1 are a cause of Paget disease of bone (PDB) [MIM:602080]. PDB is a metabolic bone disease affecting the axial skeleton and characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Manifestations of the disease include bone pain, deformity, pathological fractures, deafness, neurological complications and increased risk of osteosarcoma. PDB is a chronic disease affecting 2 to 3% of the population above the age of 40 years.
Sequence similaritiesContains 1 OPR domain.
Contains 1 UBA domain.
Contains 1 ZZ-type zinc finger.
DomainThe UBA domain binds specifically 'Lys-63'-linked polyubiquitin chains of polyubiquitinated substrates. Mediates the interaction with TRIM55.
The OPR domain mediates homooligomerization and interactions with PRKCZ, PRKCI, MAP2K5 and NBR1.
The ZZ-type zinc finger mediates the interaction with RIPK1.
modificationsPhosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN.
Cellular localizationCytoplasm. Late endosome. Nucleus. Sarcomere (By similarity). In cardiac muscles localizes to the sarcomeric band (By similarity). Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma.
- Information by UniProt
- A170 antibody
- DMRV antibody
- EBI 3 associated protein of 60 kDa antibody
Western blot analysis of SQSTM1 expression in K562 cell lysate with ab91526 at 1µg/ml in (A) the absence and (B) the presence of blocking peptide.
All lanes : Anti-SQSTM1 / p62 antibody (ab91526) at 1/1000 dilution
All lanes : Mouse gastrocnemius
Lysates/proteins at 40 µg per lane.
All lanes : Anti-Rabbit IgG (H + L) at 1/10000 dilution
Predicted band size: 47 kDa
Immunohistochemical analysis of mouse VCPR155H/R155H quadricep tissue, staining SQSTM1 / p62 (green) with ab91526.
Mice were transcardially perfused with 4% paraformaldehyde before muscle tissue was frozen and sectioned. Tissue was incubated with primary antibody before detection with a fluorescein-conjugated anti-rabbit secondary antibody. Nuclei were stained with DAPI (blue).
Paraffin-embedded human spleen tissue stained for SQSTM1/p62 using ab91526 at 5 µg/ml in immunohistochemical analysis.
Lane 1 : Anti-SQSTM1 / p62 antibody (ab91526) at 1 µg/ml
Lane 2 : Anti-SQSTM1 / p62 antibody (ab91526) at 2 µg/ml
All lanes : Human spleen tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 47 kDa
ab91526 at a 1/500 dilution staining SQSTM1/ p62 in wild type murine embryonic fibroblasts by Immunocytochemistry/ Immunofluorescence.
Immunofluorescence of SQSTM1 in Rat Spleen cells using ab91526 at 20 ug/ml.
This product has been referenced in:
- Paiva NM et al. Ileal pouch of ulcerative colitis and familial adenomatous polyposis patients exhibit modulation of autophagy markers. Sci Rep 8:2619 (2018). WB, IHC-P ; Human . Read more (PubMed: 29422639) »
- Liu W et al. Dexamethasone-induced production of reactive oxygen species promotes apoptosis via endoplasmic reticulum stress and autophagy in MC3T3-E1 cells. Int J Mol Med 41:2028-2036 (2018). Read more (PubMed: 29393368) »