Recombinant
RabMAb

Recombinant Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)

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Overview

  • Product name

    Anti-SR protein repeat/SRSF6 antibody [EPR9231]
    See all SR protein repeat/SRSF6 primary antibodies

  • Description

    Rabbit monoclonal [EPR9231] to SR protein repeat/SRSF6

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human SR protein repeat/SRSF6 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • K562, Jurkat and HeLa whole cell lysate (ab150035); Human gastric carcinoma tissue.

  • General notes

     

     

     This product was previously labelled as SR protein repeat

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9231

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab140623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Function

      Plays a role in constitutive splicing and can modulate the selection of alternative splice sites. Represses the splicing of MAPT/Tau exon 10.

    • Sequence similarities

      Belongs to the splicing factor SR family.
      Contains 2 RRM (RNA recognition motif) domains.

    • Post-translational
      modifications

      Extensively phosphorylated on serine residues in the RS domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • arginine/serine-rich 6 antibody
        • B52 antibody
        • Epididymis secretory protein Li 91 antibody
        • fc17h09 antibody
        • HEL S 91 antibody
        • MGC128807 antibody
        • MGC5045 antibody
        • Pre mRNA splicing factor SRP55 antibody
        • Pre-mRNA-splicing factor SRP55 antibody
        • Serine/arginine-rich splicing factor 6 antibody
        • SFRS6 antibody
        • Splicing factor antibody
        • Splicing factor arginine/serine rich 55 kDa antibody
        • Splicing factor arginine/serine rich 6 antibody
        • SR splicing factor 6 antibody
        • SRP55 antibody
        • SRSF6 antibody
        • SRSF6_HUMAN antibody
        • wu:faa54g02 antibody
        • wu:fc17h09 antibody
        • zgc:103497 antibody
        see all

      Images

      • Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
        Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
        All lanes : Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623) at 1/1000 dilution

        Lane 1 : K562 cell lysate
        Lane 2 : Jurkat cell lysate
        Lane 3 : HeLa cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 40 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)

        Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labelling SR protein repeat/SRSF6 with ab140623 at 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)

        Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab140623 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)

        Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab140623 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)

        Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab140623 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)

        Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab140623 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      References

      This product has been referenced in:

      See 1 Publication for this product

      Publishing research using ab140623? Please let us know so that we can cite the reference in this datasheet.

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