Recombinant Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-SR protein repeat/SRSF6 antibody [EPR9231]
See all SR protein repeat/SRSF6 primary antibodies -
Description
Rabbit monoclonal [EPR9231] to SR protein repeat/SRSF6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human SR protein repeat/SRSF6 aa 150-250. The exact sequence is proprietary.
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Positive control
- K562, Jurkat and HeLa whole cell lysate (ab150035); Human gastric carcinoma tissue.
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General notes
This product was previously labelled as SR protein repeat
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9231 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab140623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 40 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Plays a role in constitutive splicing and can modulate the selection of alternative splice sites. Represses the splicing of MAPT/Tau exon 10. -
Sequence similarities
Belongs to the splicing factor SR family.
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsExtensively phosphorylated on serine residues in the RS domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6431 Human
- Entrez Gene: 67996 Mouse
- Entrez Gene: 362264 Rat
- Omim: 601944 Human
- SwissProt: Q13247 Human
- Unigene: 6891 Human
- Unigene: 24042 Mouse
- Unigene: 3175 Rat
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Alternative names
- arginine/serine-rich 6 antibody
- B52 antibody
- Epididymis secretory protein Li 91 antibody
see all
Images
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![Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-155917-anti-sr-protein-repeat-antibody-epr9231-western-blot.jpg)
Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)All lanes : Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-155916-anti-sr-protein-repeat-antibody-epr9231-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labelling SR protein repeat/SRSF6 with ab140623 at 1/50 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-155915-anti-sr-protein-repeat-antibody-epr9231-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab140623 showing +ve staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-155914-anti-sr-protein-repeat-antibody-epr9231-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab140623 showing +ve staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-155913-anti-sr-protein-repeat-antibody-epr9231-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab140623 showing +ve staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-155912-anti-sr-protein-repeat-antibody-epr9231-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab140623 showing +ve staining.
Datasheets and documents
References
This product has been referenced in:
- Neueder A et al. Regulatory mechanisms of incomplete huntingtin mRNA splicing. Nat Commun 9:3955 (2018). Read more (PubMed: 30262848) »
