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    sr-protein-repeatsrsf6-antibody-epr9231-ab140623.pdf

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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Splicing
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)

  • Datasheet
  • SDS
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Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
  • Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
  • Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9231] to SR protein repeat/SRSF6
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

You may also be interested in

Knockout
Product image
Human SRSF6 (SR protein repeat) knockout HEK-293T cell line (ab266529)

View more associated products

Overview

  • Product name

    Anti-SR protein repeat/SRSF6 antibody [EPR9231]
    See all SR protein repeat/SRSF6 primary antibodies
  • Description

    Rabbit monoclonal [EPR9231] to SR protein repeat/SRSF6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human SR protein repeat/SRSF6 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: HEK293T, K562, Jurkat and HeLa whole cell lysates. IHC-P: Human gastric, ovarian and brain carcinoma tissue; Human uterus and brain tissue.
  • General notes

     

     

     This product was previously labelled as SR protein repeat

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9231
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing

Associated products

  • Alternative Versions

    • Anti-SR protein repeat/SRSF6 antibody [EPR9231] - BSA and Azide free (ab248910)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human SRSF6 (SR protein repeat) knockout HEK-293T cell line (ab266529)
  • KO cell lysates

    • Human SRSF6 (SR protein repeat) knockout HEK-293T cell lysate (ab258210)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
    • K-562 whole cell lysate (ab7911)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab140623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Plays a role in constitutive splicing and can modulate the selection of alternative splice sites. Represses the splicing of MAPT/Tau exon 10.
    • Sequence similarities

      Belongs to the splicing factor SR family.
      Contains 2 RRM (RNA recognition motif) domains.
    • Post-translational
      modifications

      Extensively phosphorylated on serine residues in the RS domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q13247 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6431 Human
      • Entrez Gene: 67996 Mouse
      • Entrez Gene: 362264 Rat
      • Omim: 601944 Human
      • SwissProt: Q13247 Human
      • Unigene: 6891 Human
      • Unigene: 24042 Mouse
      • Unigene: 3175 Rat
      • Alternative names

        • arginine/serine-rich 6 antibody
        • B52 antibody
        • Epididymis secretory protein Li 91 antibody
        • fc17h09 antibody
        • HEL S 91 antibody
        • MGC128807 antibody
        • MGC5045 antibody
        • Pre mRNA splicing factor SRP55 antibody
        • Pre-mRNA-splicing factor SRP55 antibody
        • Serine/arginine-rich splicing factor 6 antibody
        • SFRS6 antibody
        • Splicing factor antibody
        • Splicing factor arginine/serine rich 55 kDa antibody
        • Splicing factor arginine/serine rich 6 antibody
        • SR splicing factor 6 antibody
        • SRP55 antibody
        • SRSF6 antibody
        • SRSF6_HUMAN antibody
        • wu:faa54g02 antibody
        • wu:fc17h09 antibody
        • zgc:103497 antibody
        see all

      Images

      • Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
        Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
        All lanes : Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623) at 1/1000 dilution

        Lane 1 : Wild-type HEK293T cell lysate
        Lane 2 : SRSF6 knockout HEK293T cell lysate
        Lane 3 : Jurkat cell lysate
        Lane 4 : K-562 cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

        Predicted band size: 40 kDa
        Observed band size: 48 kDa why is the actual band size different from the predicted?



        Lanes 1-4: Merged signal (red and green). Green - ab140623 observed at 48 kDa. Red - loading control ab8245 observed at 36 kDa.

         ab140623 Anti-SR protein repeat/SRSF6 antibody [EPR9231] was shown to specifically react with SR protein repeat/SRSF6 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266529 (knockout cell lysate ab258210) was used. Wild-type and SR protein repeat/SRSF6 knockout samples were subjected to SDS-PAGE. ab140623 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)

        Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labelling SR protein repeat/SRSF6 with ab140623 at 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
        Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
        All lanes : Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623) at 1/1000 dilution

        Lane 1 : K562 cell lysate
        Lane 2 : Jurkat cell lysate
        Lane 3 : HeLa cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 40 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)

        Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab140623 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)

        Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab140623 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)

        Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab140623 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)

        Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab140623 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
        Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (2)

      Publishing research using ab140623? Please let us know so that we can cite the reference in this datasheet.

      ab140623 has been referenced in 2 publications.

      • Li M  et al. miR-193a-5p promotes pancreatic cancer cell metastasis through SRSF6-mediated alternative splicing of OGDHL and ECM1. Am J Cancer Res 10:38-59 (2020). PubMed: 32064152
      • Neueder A  et al. Regulatory mechanisms of incomplete huntingtin mRNA splicing. Nat Commun 9:3955 (2018). PubMed: 30262848

      Customer reviews and Q&As

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