Recombinant Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
![Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-397738-anti-sr-protein-repeatsrsf6-antibody-epr9231-western-blot-human-knockout-lysate.jpg "Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9231] to SR protein repeat/SRSF6
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-SR protein repeat/SRSF6 antibody [EPR9231]
See all SR protein repeat/SRSF6 primary antibodies -
Description
Rabbit monoclonal [EPR9231] to SR protein repeat/SRSF6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human SR protein repeat/SRSF6 aa 150-250. The exact sequence is proprietary.
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Positive control
- WB: HEK293T, K562, Jurkat and HeLa whole cell lysates. IHC-P: Human gastric, ovarian and brain carcinoma tissue; Human uterus and brain tissue.
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General notes
This product was previously labelled as SR protein repeat
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9231 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Plays a role in constitutive splicing and can modulate the selection of alternative splice sites. Represses the splicing of MAPT/Tau exon 10. -
Sequence similarities
Belongs to the splicing factor SR family.
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsExtensively phosphorylated on serine residues in the RS domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6431 Human
- Entrez Gene: 67996 Mouse
- Entrez Gene: 362264 Rat
- Omim: 601944 Human
- SwissProt: Q13247 Human
- Unigene: 6891 Human
- Unigene: 24042 Mouse
- Unigene: 3175 Rat
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Alternative names
- arginine/serine-rich 6 antibody
- B52 antibody
- Epididymis secretory protein Li 91 antibody
see all
Images
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Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)All lanes : Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : SRSF6 knockout HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K-562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 40 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab140623 observed at 48 kDa. Red - loading control ab8245 observed at 36 kDa.
ab140623 Anti-SR protein repeat/SRSF6 antibody [EPR9231] was shown to specifically react with SR protein repeat/SRSF6 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266529 (knockout cell lysate ab258210) was used. Wild-type and SR protein repeat/SRSF6 knockout samples were subjected to SDS-PAGE. ab140623 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-155916-anti-sr-protein-repeat-antibody-epr9231-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labelling SR protein repeat/SRSF6 with ab140623 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-155917-anti-sr-protein-repeat-antibody-epr9231-western-blot.jpg)
Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)All lanes : Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-155915-anti-sr-protein-repeat-antibody-epr9231-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab140623 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-155914-anti-sr-protein-repeat-antibody-epr9231-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab140623 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-155913-anti-sr-protein-repeat-antibody-epr9231-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab140623 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-155912-anti-sr-protein-repeat-antibody-epr9231-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab140623 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)](https://www.abcam.com/ps/products/140/ab140623/Images/ab140623-8-benefits-of-recombinant-antibodies.png)
Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab140623 has been referenced in 2 publications.
- Li M et al. miR-193a-5p promotes pancreatic cancer cell metastasis through SRSF6-mediated alternative splicing of OGDHL and ECM1. Am J Cancer Res 10:38-59 (2020). PubMed: 32064152
- Neueder A et al. Regulatory mechanisms of incomplete huntingtin mRNA splicing. Nat Commun 9:3955 (2018). PubMed: 30262848