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    sr140-antibody-ab195340.pdf

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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Splicing
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Anti-SR140 antibody (ab195340)

  • Datasheet
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Western blot - Anti-SR140 antibody (ab195340)
  • Immunoprecipitation - Anti-SR140 antibody (ab195340)

Key features and details

  • Rabbit polyclonal to SR140
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-SR140 antibody
  • Description

    Rabbit polyclonal to SR140
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human SR140 aa 1-50. The exact sequence is proprietary. NP_001073884.1
    Sequence:

    MADKTPGGSQKASSKTRSSDVHSSGSSDAHMDASGPSDSDMPSRTRPKSP


    Database link: O15042
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa, 293T, Jurkat, Mouse TCMK-1 and Mouse NIH/3T3 whole cell lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab195340 was affinity purified using an epitope specific to SR140 immobilized on a solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • NIH 3T3 whole cell lysate (ab7179)
    • Jurkat whole cell lysate (ab7899)

Applications

Our Abpromise guarantee covers the use of ab195340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 118 kDa.

Target

  • Target information above from: UniProt accession O15042 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 460749 Chimpanzee
    • Entrez Gene: 505019 Cow
    • Entrez Gene: 477103 Dog
    • Entrez Gene: 101146707 Gorilla
    • Entrez Gene: 100051188 Horse
    • Entrez Gene: 23350 Human
    • Entrez Gene: 67958 Mouse
    • Entrez Gene: 100174588 Orangutan
    • Entrez Gene: 100517309 Pig
    • Entrez Gene: 100353698 Rabbit
    • Entrez Gene: 716408 Rhesus monkey
    • Entrez Gene: 101104449 Sheep
    • SwissProt: O15042 Human
    • SwissProt: Q6NV83 Mouse
    • SwissProt: Q5R7X2 Orangutan
    • Unigene: 596572 Human
    • Unigene: 292742 Mouse
    • Unigene: 392755 Mouse
    see all
  • Alternative names

    • 140 kDa Ser/Arg rich domain protein antibody
    • 140 kDa Ser/Arg-rich domain protein antibody
    • AU023006 antibody
    • fSAPa antibody
    • Functional spliceosome associated protein a antibody
    • KIAA0332 antibody
    • MGC133197 antibody
    • Ser/Arg rich domain protein, 140 kDa antibody
    • SR140 U2 associated SR140 protein antibody
    • SR140_HUMAN antibody
    • U2 associated SR140 protein antibody
    • U2 snRNP associated SURP domain containing antibody
    • U2 snRNP-associated SURP motif-containing protein antibody
    • U2-associated protein SR140 antibody
    • U2SURP antibody
    see all

Images

  • Western blot - Anti-SR140 antibody (ab195340)
    Western blot - Anti-SR140 antibody (ab195340)
    All lanes : Anti-SR140 antibody (ab195340) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate prepared using NETN lysis buffer
    Lane 2 : 293T whole cell lysate prepared using NETN lysis buffer
    Lane 3 : Jurkat whole cell lysate prepared using NETN lysis buffer
    Lane 4 : Mouse TCMK-1 whole cell lysate prepared using NETN lysis buffer
    Lane 5 : Mouse NIH/3T3 whole cell lysate prepared using NETN lysis buffer

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 118 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation - Anti-SR140 antibody (ab195340)
    Immunoprecipitation - Anti-SR140 antibody (ab195340)

    Detection of SR140 in Immunoprecipitates of 293T whole cell lysates prepared
    using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab195340 at 6 µg per reaction for IP and at 0.1µg/ml for subsequent Western blot detection.
    Detection: Chemiluminescence with an exposure time of 10 seconds.

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab195340? Please let us know so that we can cite the reference in this datasheet.

    ab195340 has not yet been referenced specifically in any publications.

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