Key features and details
- Rabbit polyclonal to SR140
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SR140 antibody
DescriptionRabbit polyclonal to SR140
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- HeLa, 293T, Jurkat, Mouse TCMK-1 and Mouse NIH/3T3 whole cell lysates.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab195340 was affinity purified using an epitope specific to SR140 immobilized on a solid support.
Our Abpromise guarantee covers the use of ab195340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 118 kDa.|
- Information by UniProt
- 140 kDa Ser/Arg rich domain protein antibody
- 140 kDa Ser/Arg-rich domain protein antibody
- AU023006 antibody
All lanes : Anti-SR140 antibody (ab195340) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate prepared using NETN lysis buffer
Lane 2 : 293T whole cell lysate prepared using NETN lysis buffer
Lane 3 : Jurkat whole cell lysate prepared using NETN lysis buffer
Lane 4 : Mouse TCMK-1 whole cell lysate prepared using NETN lysis buffer
Lane 5 : Mouse NIH/3T3 whole cell lysate prepared using NETN lysis buffer
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 118 kDa
Exposure time: 30 seconds
Detection of SR140 in Immunoprecipitates of 293T whole cell lysates prepared
using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab195340 at 6 µg per reaction for IP and at 0.1µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab195340 has not yet been referenced specifically in any publications.