Overview

  • Product name
    Anti-SRA1 antibody [EPR11776]
    See all SRA1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11776] to SRA1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SRA1 aa 1-100. The exact sequence is proprietary.
    Database link: Q9HD15

  • Positive control
    • Human fetal liver and kidney lysates; Human ovarian carcinoma tissue; 293 and MCF7 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
Flow Cyt 1/180.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 26 kDa).

Target

  • Function
    Functional RNA which acts as a transcriptional coactivator that selectively enhances steroid receptor-mediated transactivation ligand-independently through a mechanism involving the modulating N-terminal domain (AF-1) of steroid receptors. Also mediates transcriptional coactivation of steroid receptors ligand-dependently through the steroid-binding domain (AF-2). Enhances cellular proliferation and differentiation and promotes apoptosis in vivo. May play a role in tumorigenesis.
  • Tissue specificity
    Highly expressed in liver and skeletal muscle and to a lesser extent in brain. Also expressed in both normal and tumorigenic breast epithelial cell lines. Significantly up-regulated in human tumors of the breast, ovary, and uterus.
  • Sequence similarities
    Belongs to the SRA1 family.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC87674 antibody
    • PP7684 antibody
    • SR A1 antibody
    • SRA antibody
    • SRA1 antibody
    • SRA1_HUMAN antibody
    • SRAP antibody
    • Steroid receptor coactivator antibody
    • Steroid receptor RNA activator 1 (complexes with NCOA1) antibody
    • Steroid receptor RNA activator 1 antibody
    • Steroid receptor RNA activator protein antibody
    • STRAA1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SRA1 with ab183725 at 1/100 dilution. The slide is counterstained with Hematoxylin.

  • Immunofluorescence analysis of 4% paraformaldehyde-fixed MCF7 cells labeling SRA1 with ab183725 at 1/500 dilution (red). Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody. The slide on the right is stained with Dapi (blue).

  • Anti-SRA1 antibody [EPR11776] (ab183725) at 1/2000 dilution + Human fetal liver lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 26 kDa

  • Anti-SRA1 antibody [EPR11776] (ab183725) at 1/10000 dilution + Human fetal kidney lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

    Predicted band size: 26 kDa

  • Flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling SRA1 with ab183725 at 1/180 dilution. Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.

References

This product has been referenced in:
  • Xie Z  et al. Adipose-Derived Exosomes Exert Proatherogenic Effects by Regulating Macrophage Foam Cell Formation and Polarization. J Am Heart Assoc 7:N/A (2018). Read more (PubMed: 29502100) »
  • Zhang X  et al. Human Gingiva-Derived Mesenchymal Stem Cells Modulate Monocytes/Macrophages and Alleviate Atherosclerosis. Front Immunol 9:878 (2018). Read more (PubMed: 29760701) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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