Recombinant Anti-SRAP antibody [EPR11776] (ab183725)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11776] to SRAP
- Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SRAP antibody [EPR11776]
See all SRAP primary antibodies -
Description
Rabbit monoclonal [EPR11776] to SRAP -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver and kidney lysates; Human ovarian carcinoma tissue; 293 and MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11776 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/180.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/500.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 26 kDa).
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Notes |
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Flow Cyt (Intra)
1/180. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/500. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 26 kDa). |
Target
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Function
Functional RNA which acts as a transcriptional coactivator that selectively enhances steroid receptor-mediated transactivation ligand-independently through a mechanism involving the modulating N-terminal domain (AF-1) of steroid receptors. Also mediates transcriptional coactivation of steroid receptors ligand-dependently through the steroid-binding domain (AF-2). Enhances cellular proliferation and differentiation and promotes apoptosis in vivo. May play a role in tumorigenesis. -
Tissue specificity
Highly expressed in liver and skeletal muscle and to a lesser extent in brain. Also expressed in both normal and tumorigenic breast epithelial cell lines. Significantly up-regulated in human tumors of the breast, ovary, and uterus. -
Sequence similarities
Belongs to the SRA1 family. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10011 Human
- Entrez Gene: 24068 Mouse
- Entrez Gene: 252891 Rat
- Omim: 603819 Human
- SwissProt: Q9HD15 Human
- SwissProt: Q80VJ2 Mouse
- SwissProt: Q6QGW5 Rat
- Unigene: 653135 Human
see all -
Alternative names
- MGC87674 antibody
- PP7684 antibody
- SR A1 antibody
see all
Images
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Anti-SRAP antibody [EPR11776] (ab183725) at 1/2000 dilution + Human fetal liver lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 26 kDa -
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SRAP with ab183725 at 1/100 dilution. The slide is counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunofluorescence analysis of 4% paraformaldehyde-fixed MCF7 cells labeling SRAP with ab183725 at 1/500 dilution (red). Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody. The slide on the right is stained with Dapi (blue).
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Anti-SRAP antibody [EPR11776] (ab183725) at 1/10000 dilution + Human fetal kidney lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated
Predicted band size: 26 kDa -
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling SRAP with ab183725 at 1/180 dilution. Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (8)
ab183725 has been referenced in 8 publications.
- Cokic I et al. Intramyocardial hemorrhage drives fatty degeneration of infarcted myocardium. Nat Commun 13:6394 (2022). PubMed: 36302906
- Liu W et al. Recombinant Klotho protein enhances cholesterol efflux of THP-1 macrophage-derived foam cells via suppressing Wnt/ß-catenin signaling pathway. BMC Cardiovasc Disord 20:120 (2020). PubMed: 32138681
- Hong CH et al. Steroid Receptor RNA Activator, a Long Noncoding RNA, Activates p38, Facilitates Epithelial-Mesenchymal Transformation, and Mediates Experimental Melanoma Metastasis. J Invest Dermatol 140:1355-1363.e1 (2020). PubMed: 31945347
- Gu X et al. An in vitro model of foam cell formation induced by a stretchable microfluidic device. Sci Rep 9:7461 (2019). PubMed: 31097769
- Zhong S et al. Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation. J Clin Invest 129:252-267 (2019). PubMed: 30375985
- Wang Z et al. Naoxintong Retards Atherosclerosis by Inhibiting Foam Cell Formation Through Activating Ppara Pathway. Curr Mol Med 18:698-710 (2018). PubMed: 30734676
- Xie Z et al. Adipose-Derived Exosomes Exert Proatherogenic Effects by Regulating Macrophage Foam Cell Formation and Polarization. J Am Heart Assoc 7:N/A (2018). PubMed: 29502100
- Zhang X et al. Human Gingiva-Derived Mesenchymal Stem Cells Modulate Monocytes/Macrophages and Alleviate Atherosclerosis. Front Immunol 9:878 (2018). PubMed: 29760701