For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    srankl-antibody-ab62516.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Innate Immunity Cytokines TNF Superfamily
Share by email

Anti-sRANKL antibody (ab62516)

  • Datasheet
Reviews (6)Q&A (2)References (14)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-sRANKL antibody (ab62516)
  • Western blot - Anti-sRANKL antibody (ab62516)
  • Western blot - Anti-sRANKL antibody (ab62516)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-sRANKL antibody (ab62516)

Key features and details

  • Rabbit polyclonal to sRANKL
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human sRANKL protein (ab108129)
Primary
Product image
Anti-Osteoprotegerin antibody (ab203061)
ELISA
Product image
Human TNFSF11 ELISA Kit (RANKL) (ab213841)

View more associated products

Overview

  • Product name

    Anti-sRANKL antibody
    See all sRANKL primary antibodies
  • Description

    Rabbit polyclonal to sRANKL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) of 14 amino acids from near the centre of sRANKL

  • General notes

    This antibody will recognize both the soluble form and the uncleaved transmembrane form of RANK-L.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone
    • Cardiovascular
    • Atherosclerosis
    • Thrombosis
    • Other
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human sRANKL protein (ab108129)

Applications

Our Abpromise guarantee covers the use of ab62516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 35 kDa).
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
  • Tissue specificity

    Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
  • Involvement in disease

    Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development.
  • Sequence similarities

    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications

    The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.
  • Cellular localization

    Cytoplasm; Secreted and Cell membrane.
  • Target information above from: UniProt accession O14788 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8600 Human
    • Entrez Gene: 21943 Mouse
    • Entrez Gene: 117516 Rat
    • Omim: 602642 Human
    • SwissProt: O14788 Human
    • SwissProt: O35235 Mouse
    • SwissProt: Q9ESE2 Rat
    • Unigene: 333791 Human
    • Unigene: 249221 Mouse
    • Unigene: 217570 Rat
    • Unigene: 64517 Rat
    see all
  • Alternative names

    • CD254 antibody
    • hRANKL2 antibody
    • ODF antibody
    • OPGL antibody
    • OPTB2 antibody
    • Osteoclast differentiation factor antibody
    • Osteoprotegerin ligand antibody
    • RANKL antibody
    • Receptor activator of NF-kappa-B ligand antibody
    • Receptor activator of nuclear factor kappa B ligand antibody
    • Receptor activator of nuclear factor kappa-B ligand antibody
    • sOdf antibody
    • soluble form antibody
    • TNF-related activation-induced cytokine antibody
    • TNF11_HUMAN antibody
    • Tnfsf11 antibody
    • TRANCE antibody
    • Tumor necrosis factor (ligand) superfamily member 11 antibody
    • Tumor necrosis factor ligand superfamily member 11 antibody
    see all

Images

  • Western blot - Anti-sRANKL antibody (ab62516)
    Western blot - Anti-sRANKL antibody (ab62516)
    All lanes : Anti-sRANKL antibody (ab62516) at 1 µg/ml

    Lane 1 : A431 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

    Predicted band size: 35 kDa

  • Western blot - Anti-sRANKL antibody (ab62516)
    Western blot - Anti-sRANKL antibody (ab62516)
    All lanes : Anti-sRANKL antibody (ab62516) at 2 µg/ml

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Mouse skin tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

    Predicted band size: 35 kDa

  • Western blot - Anti-sRANKL antibody (ab62516)
    Western blot - Anti-sRANKL antibody (ab62516)
    Lane 1 : Anti-sRANKL antibody (ab62516) at 0.25 µg/ml
    Lane 2 : Anti-sRANKL antibody (ab62516) at 0.5 µg/ml

    All lanes : rat liver tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 35 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-sRANKL antibody (ab62516)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-sRANKL antibody (ab62516)
    Immunohistochemical analysis of RANKL expression in paraffin embedded human liver tissue using 5 µg/ml ab62516

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (14)

    Publishing research using ab62516? Please let us know so that we can cite the reference in this datasheet.

    ab62516 has been referenced in 14 publications.

    • Gama A  et al. Origins of Alterations to Rankl Null Mutant Mouse Dental Root Development. Int J Mol Sci 21:N/A (2020). PubMed: 32209985
    • Shu Q  et al. Mechanisms by which electroacupuncture-mediated histone acetylation mitigates bone loss in rats with ovariectomy-induced osteoporosis. Mol Med Rep 22:3453-3463 (2020). PubMed: 32945471
    • Ock S  et al. RANKL blockade suppresses pathological angiogenesis and vascular leakage in ischemic retinopathy. Biochem Biophys Res Commun 516:350-356 (2019). PubMed: 31208720
    • Hiyama S  et al. Osteoclastogenesis from bone marrow cells during estrogen-induced medullary bone formation in Japanese quails. J Mol Histol 50:389-404 (2019). PubMed: 31214852
    • Tao YW  et al. Association between Increased Inducible Costimulator/Inducible Costimulator Ligand Expression with Bone Destruction in Apical Periodontitis. J Endod 45:890-897 (2019). PubMed: 31153660
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-8 of 8 Abreviews or Q&A

    Western blot abreview for Anti-sRANKL antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    36 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Rat Tissue lysate - whole (Bone - Tibia)
    Specification
    Bone - Tibia
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Mr. Helder Fonseca

    Verified customer

    Submitted Sep 25 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-sRANKL antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    PBS BSA as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
    Antigen retrieval step
    None
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 21 2014

    ELISA abreview for Anti-sRANKL antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Cell (cell culture supernatants (osteoblast))
    Specification
    cell culture supernatants (osteoblast)
    Type
    Sandwich (Capture)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 09 2013

    Western blot abreview for Anti-sRANKL antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (5-16% gels)
    Sample
    Human Tissue lysate - whole (skin)
    Specification
    skin
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 03 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-sRANKL antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Sample
    Human Cell (HeLa cells)
    Specification
    HeLa cells
    Permeabilization
    Yes - 0,1% Saponin/PBS
    Fixative
    Methanol
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 28 2013

    Question

    One of our customers is looking for an anti-RANKL antibody to be used for IHC-P, rat tissues. So he asks:

    1- Is there any ABCAM anti-RANKL antibody which only detects transmembrane forms of the protein?

    2- Can ab62516 (raised against soluble RANKL protein) also detect the transmembrane forms of the protein?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 20 2012

    Answer

    Thank you for your interest in our products.

    Currently we do not carry an anti-RANKL antibody targeting the transmembrane portion of the protein. However, we are happy to be able to offer custom antibody services through Epitomics, an Abcam company. For more information about these services visit:

    http://www.epitomics.com/services/
    email: service@epitomics.com

    Alternatively, please contact Li Li at Epitomics:

    li.li@epitomics.com

    For ab62516, this does not target the transmembrane form of the protein. The sequence it targets is outside a.a. 48 - 68.

    I hope this information is helpful to you. Please do not hesitate to contact us if you have any questions.

    Read More

    Abcam Scientific Support

    Answered on Dec 20 2012

    Question

    Can you provide the immunogen sequence for this antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 14 2012

    Answer

    Thank you for contacting Abcam.

    The immunogen sequence for ab62516 is **************.

    Please let me know if there is anything else I can help you with.

    Read More

    Abcam Scientific Support

    Answered on Mar 14 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-sRANKL antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (lung)
    Specification
    lung
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
    Permeabilization
    Yes - 0,1% Saponin / PBS
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 27 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.