- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-sRANKL antibody
See all sRANKL primary antibodies -
DescriptionRabbit polyclonal to sRANKL
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, ELISA, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide (Human) of 14 amino acids from near the centre of sRANKL
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Positive control
- Rat liver tissue lysate Human liver tissue
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General notes
This antibody will recognize both the soluble form and the uncleaved transmembrane form of RANK-L.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
Applications
Our Abpromise guarantee covers the use of ab62516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 35 kDa). | |
| ICC/IF | Use a concentration of 1 µg/ml. | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 5 µg/ml. |
Target
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FunctionCytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
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Tissue specificityHighest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
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Involvement in diseaseDefects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development.
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Sequence similaritiesBelongs to the tumor necrosis factor family.
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Post-translational
modificationsThe soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17. -
Cellular localizationCytoplasm; Secreted and Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 8600 Human
- Entrez Gene: 21943 Mouse
- Entrez Gene: 117516 Rat
- Omim: 602642 Human
- SwissProt: O14788 Human
- SwissProt: O35235 Mouse
- SwissProt: Q9ESE2 Rat
- Unigene: 333791 Human
see all -
Alternative names
- CD254 antibody
- hRANKL2 antibody
- ODF antibody
see all
Images
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Western blot - Anti-sRANKL antibody (ab62516)Lane 1 : Anti-sRANKL antibody (ab62516) at 0.25 µg/ml
Lane 2 : Anti-sRANKL antibody (ab62516) at 0.5 µg/ml
All lanes : rat liver tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 35 kDa
Observed band size: 30 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-sRANKL antibody (ab62516)Immunohistochemical analysis of RANKL expression in paraffin embedded human liver tissue using 5 µg/ml ab62516 -
Immunocytochemistry/ Immunofluorescence - Anti-sRANKL antibody (ab62516)ICC/IF image of ab62516 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62516, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References
This product has been referenced in:
- Liu F et al. Effect of Zuoguiwan on osteoporosis in ovariectomized rats through RANKL/OPG pathway mediated by ß2AR. Biomed Pharmacother 103:1052-1060 (2018). Rat . Read more (PubMed: 29710663) »
- Filion TM et al. Elastomeric osteoconductive synthetic scaffolds with acquired osteoinductivity expedite the repair of critical femoral defects in rats. Tissue Eng Part A 17:503-11 (2011). IHC-P ; Rat . Read more (PubMed: 20818999) »
Customer reviews and Q&As
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