Key features and details
- Rabbit polyclonal to sRANKL (Biotin)
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-sRANKL antibody (Biotin)
See all sRANKL primary antibodies
DescriptionRabbit polyclonal to sRANKL (Biotin)
Tested applicationsSuitable for: Sandwich ELISA, WBmore details
Species reactivityReacts with: Human
Highly pure (>98%) recombinant human sRANKL.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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FormLyophilized:Centrifuge vial prior to opening. Reconstitute in 50 µl sterile PBS containing 0.1% BSA to give a concentration of 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab83145 is sterile filtered. ab83145 was purified by affinity chromatography and then biotinylated.
Our Abpromise guarantee covers the use of ab83145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.
Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human sRANKL.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 28 kDa.
The detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non reducing conditions.
FunctionCytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
Tissue specificityHighest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.
Involvement in diseaseDefects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development.
Sequence similaritiesBelongs to the tumor necrosis factor family.
modificationsThe soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.
Cellular localizationCytoplasm; Secreted and Cell membrane.
- Information by UniProt
- CD254 antibody
- hRANKL2 antibody
- ODF antibody
To detect human sRANKL by Western Blot analysis ab83145 can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human sRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is non-reducing conditions; Human recombinant sRANKL protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 human recombinant sRANKL protein.
To detect human sRANKL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83145 is required. This biotinylated polyclonal antibody, in conjunction with an anti-RANKL (ab9957) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human sRANKL.
ab83145 has not yet been referenced specifically in any publications.