Key features and details
- Rabbit polyclonal to SRAP
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SRAP antibody
See all SRAP primary antibodies
DescriptionRabbit polyclonal to SRAP
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human SRAP (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: Q9HD15
- IHC-P: Human skeletal muscle and liver tissues. WB: HEK-293T, NS-1 and PC-12 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
Our Abpromise guarantee covers the use of ab230510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 26 kDa.|
FunctionFunctional RNA which acts as a transcriptional coactivator that selectively enhances steroid receptor-mediated transactivation ligand-independently through a mechanism involving the modulating N-terminal domain (AF-1) of steroid receptors. Also mediates transcriptional coactivation of steroid receptors ligand-dependently through the steroid-binding domain (AF-2). Enhances cellular proliferation and differentiation and promotes apoptosis in vivo. May play a role in tumorigenesis.
Tissue specificityHighly expressed in liver and skeletal muscle and to a lesser extent in brain. Also expressed in both normal and tumorigenic breast epithelial cell lines. Significantly up-regulated in human tumors of the breast, ovary, and uterus.
Sequence similaritiesBelongs to the SRA1 family.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- MGC87674 antibody
- PP7684 antibody
- SR A1 antibody
All lanes : Anti-SRAP antibody (ab230510) at 1/500 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : NS-1 cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate
Predicted band size: 26 kDa
Formalin-fixed, paraffin-embedded human liver tissue stained for SRAP using ab230510 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for SRAP using ab230510 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230510 has not yet been referenced specifically in any publications.