RecombinantRabMAb

Recombinant Anti-SRAP antibody [EPR11776] - BSA and Azide free (ab240962)

Submit a review Submit a question
Flow Cytometry - Anti-SRAP antibody [EPR11776] - BSA and Azide free (ab240962)
  • Immunocytochemistry/ Immunofluorescence - Anti-SRAP antibody [EPR11776] - BSA and Azide free (ab240962)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRAP antibody [EPR11776] - BSA and Azide free (ab240962)
  • Anti-SRAP antibody [EPR11776] - BSA and Azide free (ab240962)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11776] to SRAP - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SRAP antibody [EPR11776] - BSA and Azide free
    See all SRAP primary antibodies

  • Description

    Rabbit monoclonal [EPR11776] to SRAP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human SRAP aa 1-100. The exact sequence is proprietary.
    Database link: Q9HD15

  • Positive control

    • IHC-P: Human ovarian carcinoma tissue. Flow Cyt: 293 cells. ICC/IF: MCF7 cells.

  • General notes

    Ab240962 is the carrier-free version of ab183725. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240962 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab240962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
WB Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 26 kDa).

Target

  • Function

    Functional RNA which acts as a transcriptional coactivator that selectively enhances steroid receptor-mediated transactivation ligand-independently through a mechanism involving the modulating N-terminal domain (AF-1) of steroid receptors. Also mediates transcriptional coactivation of steroid receptors ligand-dependently through the steroid-binding domain (AF-2). Enhances cellular proliferation and differentiation and promotes apoptosis in vivo. May play a role in tumorigenesis.

  • Tissue specificity

    Highly expressed in liver and skeletal muscle and to a lesser extent in brain. Also expressed in both normal and tumorigenic breast epithelial cell lines. Significantly up-regulated in human tumors of the breast, ovary, and uterus.

  • Sequence similarities

    Belongs to the SRA1 family.

  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • MGC87674 antibody
    • PP7684 antibody
    • SR A1 antibody
    • SRA antibody
    • SRA1 antibody
    • SRA1_HUMAN antibody
    • SRAP antibody
    • Steroid receptor coactivator antibody
    • Steroid receptor RNA activator 1 (complexes with NCOA1) antibody
    • Steroid receptor RNA activator 1 antibody
    • Steroid receptor RNA activator protein antibody
    • STRAA1 antibody
    see all

Images

  • Flow Cytometry - Anti-SRAP antibody [EPR11776] - BSA and Azide free (ab240962)
    Flow Cytometry - Anti-SRAP antibody [EPR11776] - BSA and Azide free (ab240962)

    Flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling SRAP with ab183725 at 1/180 dilution. Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183725).

  • Immunocytochemistry/ Immunofluorescence - Anti-SRAP antibody [EPR11776] - BSA and Azide free (ab240962)
    Immunocytochemistry/ Immunofluorescence - Anti-SRAP antibody [EPR11776] - BSA and Azide free (ab240962)

    Immunofluorescence analysis of 4% paraformaldehyde-fixed MCF7 cells labeling SRAP with ab183725 at 1/500 dilution (red). Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody. The slide on the right is stained with Dapi (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183725).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRAP antibody [EPR11776] - BSA and Azide free (ab240962)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRAP antibody [EPR11776] - BSA and Azide free (ab240962)

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SRAP with ab183725 at 1/100 dilution. The slide is counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183725).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-SRAP antibody [EPR11776] - BSA and Azide free (ab240962)
    Anti-SRAP antibody [EPR11776] - BSA and Azide free (ab240962)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab240962? Please let us know so that we can cite the reference in this datasheet.

ab240962 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab240962.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com