SRB Assay / Sulforhodamine B Assay Kit (ab235935)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells
Overview
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Product name
SRB Assay / Sulforhodamine B Assay Kit -
Detection method
Colorimetric -
Sample type
Adherent cells -
Assay type
Cell-based (quantitative) -
Product overview
SRB Assay / Sulforhodamine B Assay Kit ab235935 relies on the stoichiometric binding of SRB (Sulforhodamine B) dye to proteins under mild acidic conditions and its subsequent extraction under basic conditions. The amount of dye extracted is a proxy for cell mass and thus the number of cells in a sample.
The measurement of the number of cells in the assay allows its use for analysis of cell viability, cytotoxicity and cell proliferation.
In the assay, cultured cells are fixed on plates, stained with Sulforhodamine B, washed and dried, then the bound dye is solubilized and the absorbance of the dye in solution is measured at OD 565 nm.
Review our cell health assay guide to learn about our kits to perform a cell viability assay, cytotoxicity assay or cell proliferation assay.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K943 Sulforhodamine B Cell Cytotoxicity Assay Kit (Colorimetric). K943-1000 is the same size as the 1000 test size of ab235935.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1000 tests 10X Solubilization Solution 1 x 22ml 20X Wash Buffer 1 x 50ml Doxorubicin 1 x 100µl Fixation Solution II 1 x 55ml SRB Solution 1 x 50ml
Images
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab235935 has been referenced in 8 publications.
- Adams LM et al. Mapping the KRAS proteoform landscape in colorectal cancer identifies truncated KRAS4B that decreases MAPK signaling. J Biol Chem 299:102768 (2023). PubMed: 36470426
- Nuzzo G et al. Identification of the Marine Alkaloid Lepadin A as Potential Inducer of Immunogenic Cell Death. Biomolecules 12:N/A (2022). PubMed: 35204747
- Chen TH et al. Oligosaccharides Ameliorate Acute Kidney Injury by Alleviating Cluster of Differentiation 44-Mediated Immune Responses in Renal Tubular Cells. Nutrients 14:N/A (2022). PubMed: 35215410
- Wnorowski A et al. Deprogramming metabolism in pancreatic cancer with a bi-functional GPR55 inhibitor and biased ß2 adrenergic agonist. Sci Rep 12:3618 (2022). PubMed: 35256673
- Sun J et al. Tideglusib promotes wound healing in aged skin by activating PI3K/Akt pathway. Stem Cell Res Ther 13:269 (2022). PubMed: 35729652
- Quach H et al. Nano-Lipids Based on Ginger Oil and Lecithin as a Potential Drug Delivery System. Pharmaceutics 14:N/A (2022). PubMed: 36015280
- Gallo C et al. A New Bioassay Platform Design for the Discovery of Small Molecules with Anticancer Immunotherapeutic Activity. Mar Drugs 18:N/A (2020). PubMed: 33260400
- Chu CW et al. Thioridazine Enhances P62-Mediated Autophagy and Apoptosis Through Wnt/ß-Catenin Signaling Pathway in Glioma Cells. Int J Mol Sci 20:N/A (2019). PubMed: 30678307