Overview

  • Product name

    SRB Assay / Sulforhodamine B Assay Kit
  • Detection method

    Colorimetric
  • Sample type

    Adherent cells
  • Assay type

    Cell-based (quantitative)
  • Product overview

    SRB Assay / Sulforhodamine B Assay Kit ab235935 relies on the stoichiometric binding of SRB (Sulforhodamine B) dye to proteins under mild acidic conditions and its subsequent extraction under basic conditions. The amount of dye extracted is a proxy for cell mass and thus the number of cells in a sample.


    The measurement of the number of cells in the assay allows its use for analysis of cell viability, cytotoxicity and cell proliferation.


    In the assay, cultured cells are fixed on plates, stained with Sulforhodamine B, washed and dried, then the bound dye is solubilized and the absorbance of the dye in solution is measured at OD 565 nm.


    Review our cell health assay guide to learn about our kits to perform a cell viability assaycytotoxicity assay or cell proliferation assay.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1000 tests
    10X Solubilization Solution 1 x 22ml
    20 mM Doxorubicin  1 x 100µl
    20X Washing Solution  1 x 50ml
    Fixation Solution 1 x 55ml
    SRB Dye Solution 1 x 50ml

Images

  • Dose-response curve of HepG2 (a), MCF7 (b) and HEK-293 (c) cells, after exposure to doxorubicin for 72 hours, as determined using the Sulforhodamine B Cell Cytotoxicity Assay Kit (Colorimetric). Assays were performed according to the kit protocol in triplicate.

Protocols

References

This product has been referenced in:

  • Chu CW  et al. Thioridazine Enhances P62-Mediated Autophagy and Apoptosis Through Wnt/ß-Catenin Signaling Pathway in Glioma Cells. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30678307) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab235935.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up