Overview

  • Product name

    Anti-SRB4 antibody
  • Description

    Rabbit polyclonal to SRB4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ChIP, WB, ELISAmore details
  • Species reactivity

    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant full length protein (Saccharomyces cerevisiae), purified by chromatography.

  • Positive control

    • Saccharomyces cerevisiae extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab63812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB 1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 78 kDa).
ELISA Use at an assay dependent dilution.

Target

  • Function

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.
  • Sequence similarities

    Belongs to the Mediator complex subunit 17 family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

    • Alternative names

      • MED17 antibody
      • MED17_YEAST antibody
      • Mediator complex subunit 17 antibody
      • Mediator of RNA polymerase II transcription subunit 17 antibody
      • SRB4 antibody
      • Suppressor of RNA polymerase B 4 antibody
      see all

    Images

    • All lanes : Anti-SRB4 antibody (ab63812) at 1/5000 dilution

      Lane 1 : Molecular weight markers
      Lane 2 : Saccharomyces cerevisiae extract

      Predicted band size: 78 kDa
      Observed band size: 100 kDa
      why is the actual band size different from the predicted?

    • ab63812 used in ChIP.
      Whole cell lysate from yeast.
      X-ChIP cross linking performed for 15 minutes using 1% formaldehyde.
      Real time PCR was used as the detection step.
      Positive control CHA1 gene.
      Negative control SPO20 gene and a nontranscribed region on ChrV.
      ab63812 used undiluted for 16 hours at 4°C.

      See Abreview

    References

    This product has been referenced in:

    • Young CP  et al. Distinct histone methylation and transcription profiles are established during the development of cellular quiescence in yeast. BMC Genomics 18:107 (2017). WB ; Saccharomyces cerevisiae . Read more (PubMed: 28122508) »
    • Paul E  et al. Genome-wide association of mediator and RNA polymerase II in wild-type and mediator mutant yeast. Mol Cell Biol 35:331-42 (2015). ChIP ; Saccharomyces cerevisiae . Read more (PubMed: 25368384) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Abreviews
    Application
    ChIP
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (YEAST)
    Specification
    YEAST
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde 1%
    Detection step
    Real-time PCR
    Positive control
    CHA1 gene
    Negative control
    SPO20 gene and a nontranscribed region on ChrV

    Dr. Suraiya Ansari

    Verified customer

    Submitted Feb 10 2012

    Answer

    DISCOUNT CODE: *** Expiration date: [April 15, 2012 This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab63812 in ChIP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

    Read More

    Question
    Answer

    Thank you for your call today and for your interest in ab63812. Since ab63812 has not been tested in ChIP, I can offer a discount off a future purchase if you buy ab63812 now, test it in ChIP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be worth 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab63812 in ChIP. I will then send a discount code. This code must be issued before purchasing ab63812 so please wait for my reply before ordering. 2. Purchase ab63812 either by phone, fax, or online (www.abcam.com). 3. Test it in ChIP. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab63812 turns out to be unsuitable for ChIP, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.  

    Read More

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