Product nameAnti-SRB4 antibody
DescriptionRabbit polyclonal to SRB4
Tested applicationsSuitable for: ChIP, WB, ELISAmore details
Species reactivityReacts with: Saccharomyces cerevisiae
Recombinant full length protein (Saccharomyces cerevisiae), purified by chromatography.
- Saccharomyces cerevisiae extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: Whole serum
Our Abpromise guarantee covers the use of ab63812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|WB||1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 78 kDa).|
|ELISA||Use at an assay dependent dilution.|
FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.
Sequence similaritiesBelongs to the Mediator complex subunit 17 family.
- Information by UniProt
- MED17 antibody
- MED17_YEAST antibody
- Mediator complex subunit 17 antibody
All lanes : Anti-SRB4 antibody (ab63812) at 1/5000 dilution
Lane 1 : Molecular weight markers
Lane 2 : Saccharomyces cerevisiae extract
Predicted band size: 78 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
ab63812 used in ChIP.
Whole cell lysate from yeast.X-ChIP cross linking performed for 15 minutes using 1% formaldehyde.
Real time PCR was used as the detection step.Positive control CHA1 gene.
Negative control SPO20 gene and a nontranscribed region on ChrV.ab63812 used undiluted for 16 hours at 4°C.
This product has been referenced in:
- Young CP et al. Distinct histone methylation and transcription profiles are established during the development of cellular quiescence in yeast. BMC Genomics 18:107 (2017). WB ; Saccharomyces cerevisiae . Read more (PubMed: 28122508) »
- Paul E et al. Genome-wide association of mediator and RNA polymerase II in wild-type and mediator mutant yeast. Mol Cell Biol 35:331-42 (2015). ChIP ; Saccharomyces cerevisiae . Read more (PubMed: 25368384) »