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    srb4-antibody-ab63812.pdf

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Epigenetics and Nuclear Signaling Transcription Other factors
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Anti-SRB4 antibody (ab63812)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)References (3)

Product price, shipping and contact information

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Abpromise

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Find out more.

Western blot - Anti-SRB4 antibody (ab63812)

    Key features and details

    • Rabbit polyclonal to SRB4
    • Suitable for: WB
    • Reacts with: Saccharomyces cerevisiae
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-SRB4 antibody
    • Description

      Rabbit polyclonal to SRB4
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Saccharomyces cerevisiae
    • Immunogen

      Recombinant full length protein (Saccharomyces cerevisiae), purified by chromatography.

    • Positive control

      • Saccharomyces cerevisiae extract.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 6
      Preservative: 0.1% Sodium azide
      Constituent: Whole serum
    • Concentration information loading...
    • Purity

      Whole antiserum
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Other factors
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Transcription Factors

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    Our Abpromise guarantee covers the use of ab63812 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 78 kDa).

    Target

    • Function

      Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.
    • Sequence similarities

      Belongs to the Mediator complex subunit 17 family.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P32569 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 856743 Saccharomyces cerevisiae
      • SwissProt: P32569 Saccharomyces cerevisiae
      • Alternative names

        • MED17 antibody
        • MED17_YEAST antibody
        • Mediator complex subunit 17 antibody
        • Mediator of RNA polymerase II transcription subunit 17 antibody
        • SRB4 antibody
        • Suppressor of RNA polymerase B 4 antibody
        see all

      Images

      • Western blot - Anti-SRB4 antibody (ab63812)
        Western blot - Anti-SRB4 antibody (ab63812)
        All lanes : Anti-SRB4 antibody (ab63812) at 1/5000 dilution

        Lane 1 : Molecular weight markers
        Lane 2 : Saccharomyces cerevisiae extract

        Predicted band size: 78 kDa
        Observed band size: 100 kDa
        why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (3)

      Publishing research using ab63812? Please let us know so that we can cite the reference in this datasheet.

      ab63812 has been referenced in 3 publications.

      • Young CP  et al. Distinct histone methylation and transcription profiles are established during the development of cellular quiescence in yeast. BMC Genomics 18:107 (2017). WB ; Saccharomyces cerevisiae . PubMed: 28122508
      • Paul E  et al. Genome-wide association of mediator and RNA polymerase II in wild-type and mediator mutant yeast. Mol Cell Biol 35:331-42 (2015). ChIP ; Saccharomyces cerevisiae . PubMed: 25368384
      • Jeronimo C & Robert F Kin28 regulates the transient association of Mediator with core promoters. Nat Struct Mol Biol 21:449-55 (2014). IP . PubMed: 24704787

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a question

      1-2 of 2 Q&A

      Question

      We spoke earlier today regarding the Abreview promotion for the srb4 antibody (ab63812).  I would like to go ahead with this program.  Please generate a discount code so that the antibody can be ordered

      Read More

      Abcam community

      Verified customer

      Asked on Dec 14 2011

      Answer

      DISCOUNT CODE: *** Expiration date: [April 15, 2012 This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab63812 in ChIP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

      Read More

      Abcam Scientific Support

      Answered on Dec 14 2011

      Question

      Interested in testing ab63812 in ChIP.

      Read More

      Abcam community

      Verified customer

      Asked on Dec 13 2011

      Answer

      Thank you for your call today and for your interest in ab63812. Since ab63812 has not been tested in ChIP, I can offer a discount off a future purchase if you buy ab63812 now, test it in ChIP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be worth 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab63812 in ChIP. I will then send a discount code. This code must be issued before purchasing ab63812 so please wait for my reply before ordering. 2. Purchase ab63812 either by phone, fax, or online (www.abcam.com). 3. Test it in ChIP. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab63812 turns out to be unsuitable for ChIP, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.  

      Read More

      Abcam Scientific Support

      Answered on Dec 13 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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