Anti-SRBC antibody (ab179923)
Key features and details
- Goat polyclonal to SRBC
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SRBC antibody
See all SRBC primary antibodies -
Description
Goat polyclonal to SRBC -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Rabbit, Horse, Hamster, Cow, Dog, Pig, Monkey, Gorilla -
Immunogen
Synthetic peptide corresponding to Human SRBC aa 179-190 (internal sequence) (Cysteine residue). NP_659477.2.
Sequence:RRTGLQKVQSLR
Database link: Q969G5 -
Positive control
- Human uterus (myometrium) tissue; Human adipose tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 28 kDa).
The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bastiani M, J Cell Biol. 2009 Jun 29;185(7):1259-73. PMID: 19546242 ). |
IHC-P |
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 28 kDa). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bastiani M, J Cell Biol. 2009 Jun 29;185(7):1259-73. PMID: 19546242 ). |
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Plays a role in the regulation of the circadian clock. Modulates the period length and phase of circadian gene expression and also regulates expression and interaction of the core clock components PER1/2 and CRY1/2. Seems to have an immune potentiation function. -
Tissue specificity
Strongly expressed in mammary and epithelial cells. -
Sequence similarities
Belongs to the PTRF/SDPR family. -
Post-translational
modificationsIn vitro, phosphorylated by PRKCD. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 510203 Cow
- Entrez Gene: 476831 Dog
- Entrez Gene: 101144390 Gorilla
- Entrez Gene: 101826946 Hamster
- Entrez Gene: 100069918 Horse
- Entrez Gene: 112464 Human
- Entrez Gene: 85332 Rat
- SwissProt: A4FV37 Cow
see all -
Alternative names
- Cavin 3 antibody
- Cavin-3 antibody
- CAVIN3 antibody
see all
Images
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Anti-SRBC antibody (ab179923) at 0.1 µg/ml + Human adipose tissue lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 28 kDaPrimary incubation was 1 hour.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus (myometrium) tissue labeling SRBC with ab179923 at 3.75 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab179923 has been referenced in 1 publication.
- Tian Y et al. Integrated Bioinformatic Analysis of the Expression and Prognosis of Caveolae-Related Genes in Human Breast Cancer. Front Oncol 11:703501 (2021). PubMed: 34513683