Anti-SRBC antibody (ab179923)

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Overview

  • Product name

    Anti-SRBC antibody
    See all SRBC primary antibodies

  • Description

    Goat polyclonal to SRBC

  • Host species

    Goat

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Hamster, Cow, Dog, Pig, Monkey, Gorilla

  • Immunogen

    Synthetic peptide corresponding to Human SRBC aa 179-190 (internal sequence) (Cysteine residue). NP_659477.2.
    Sequence:

    RRTGLQKVQSLR


    Database link: Q969G5

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human uterus (myometrium) tissue; Human adipose tissue lysate.

  • General notes

    Protein previously labeled as PRKCDBP.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab179923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 28 kDa).

The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bastiani M, J Cell Biol. 2009 Jun 29;185(7):1259-73. PMID: 19546242 ).
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Western blot - Anti-SRBC antibody (ab179923)
    Western blot - Anti-SRBC antibody (ab179923)
    Anti-SRBC antibody (ab179923) at 0.1 µg/ml + Human adipose tissue lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 28 kDa



    Primary incubation was 1 hour.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRBC antibody (ab179923)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRBC antibody (ab179923)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus (myometrium) tissue labeling SRBC with ab179923 at 3.75 µg/ml.

References

ab179923 has not yet been referenced specifically in any publications.

Publishing research using ab179923? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Western blot abreview for Anti-SRBC antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Spleen (1,2), Lung (3))
Gel Running Conditions
Reduced Denaturing (gel 10 %)
Loading amount
30 µg
Specification
Spleen (1,2), Lung (3)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Read More

Ruth Norman

Verified customer

Submitted Feb 20 2018

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