Anti-SRBC antibody (ab179923)

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Western blot - Anti-SRBC antibody (ab179923)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRBC antibody (ab179923)

Key features and details

  • Goat polyclonal to SRBC
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-SRBC antibody
    See all SRBC primary antibodies

  • Description

    Goat polyclonal to SRBC

  • Host species

    Goat

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Hamster, Cow, Dog, Pig, Monkey, Gorilla

  • Immunogen

    Synthetic peptide corresponding to Human SRBC aa 179-190 (internal sequence) (Cysteine residue). NP_659477.2.
    Sequence:

    RRTGLQKVQSLR


    Database link: Q969G5
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human uterus (myometrium) tissue; Human adipose tissue lysate.

  • General notes

    Protein previously labeled as PRKCDBP.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab179923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 28 kDa).

The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bastiani M, J Cell Biol. 2009 Jun 29;185(7):1259-73. PMID: 19546242 ).
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Western blot - Anti-SRBC antibody (ab179923)
    Western blot - Anti-SRBC antibody (ab179923)
    Anti-SRBC antibody (ab179923) at 0.1 µg/ml + Human adipose tissue lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 28 kDa



    Primary incubation was 1 hour.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRBC antibody (ab179923)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRBC antibody (ab179923)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus (myometrium) tissue labeling SRBC with ab179923 at 3.75 µg/ml.

References (0)

Publishing research using ab179923? Please let us know so that we can cite the reference in this datasheet.

ab179923 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Western blot abreview for Anti-SRBC antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Spleen (1,2), Lung (3))
Gel Running Conditions
Reduced Denaturing (gel 10 %)
Loading amount
30 µg
Specification
Spleen (1,2), Lung (3)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Read More

Ruth Norman

Verified customer

Submitted Feb 20 2018

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